Volume 8, Issue 2 (5-2019)
aumj 2019, 8(2): 116-125 |
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Naeimi S, Kaabi M, Ebadi A. Investigation of Serum Level and IL-13 Genetic Variations in Positions +2044 G/A and -1512A/C in Southern Iranian Patients with H. Pylori. aumj 2019; 8 (2) :116-125
URL: http://aums.abzums.ac.ir/article-1-923-en.html
URL: http://aums.abzums.ac.ir/article-1-923-en.html
1- Assistant Professor of Molecular Genetics, Department of Genetics, Colleague of Science, Kazerunbranch, Islamic Azad University, Kazerun, Iran , naeimis@kau.ac.ir
2- Master Science of Microbiology, Department of Microbiology, Colleague of science, Kazerunbranch,Islamic Azad University,Shiraz, Iran
3- Assistant Professor of Chemistry, Department of Chemistry, Colleague of science, Kazerunbranch,Islamic Azad University,Kazerun, Iran
2- Master Science of Microbiology, Department of Microbiology, Colleague of science, Kazerunbranch,Islamic Azad University,Shiraz, Iran
3- Assistant Professor of Chemistry, Department of Chemistry, Colleague of science, Kazerunbranch,Islamic Azad University,Kazerun, Iran
Abstract: (3311 Views)
Background: Helicobacter pylori (H. pylori)is agramnegative bacterium thatcolonizes stomach mucosa andcausesupper gastrointestinal diseases.Interleukin-13 is a preventive immune cytokine produced by many immune cells. IL-13 gene is located on chromosome 5 q 25 bp. It has polymorphisms at positions -1512 A/ C and +2044 G/A, which are associated with the expression rate of this cytokine .The aim of this study is to investigate the serum level and polymorphisms of IL-13 gene in patients with H.pylori infection.
Methods: In this case control study, DNA was isolated by salting out method from peripheral blood of 150 patients with H.pylori infection diagnosed by an expert. PCR-RFLP was used to examine polymorphisms of IL-13 gene . The results were compared with 150 healthy controls by SPSS software and Chi-square test.Tostudy, the serum levels of IL-13 cytokine, serum samples of 40 patients and 40 controls were prepared and examined using ELISA kit.
Results: According to the findings, there is a significant difference in (CI: 06/1-09/0, OR 32.0 MB) GA (P = 0/005) and (08/1-52/0: CI, OR: 45.0) GG (P = 0/045) genotypes at A/2044 G +, position between patients and the control group. No significant differences were observed at the position of -1512 A/C. Also, no significant differences were seen between serum level of IL-13 cytokine and H. pylori disease (P >. 0.05).
Conclusion: Based on the results obtained, it seems that GA and GG genotypes of interleukin-13 gene at position 2044+ make people more susceptible to H. pylori infection.
Methods: In this case control study, DNA was isolated by salting out method from peripheral blood of 150 patients with H.pylori infection diagnosed by an expert. PCR-RFLP was used to examine polymorphisms of IL-13 gene . The results were compared with 150 healthy controls by SPSS software and Chi-square test.Tostudy, the serum levels of IL-13 cytokine, serum samples of 40 patients and 40 controls were prepared and examined using ELISA kit.
Results: According to the findings, there is a significant difference in (CI: 06/1-09/0, OR 32.0 MB) GA (P = 0/005) and (08/1-52/0: CI, OR: 45.0) GG (P = 0/045) genotypes at A/2044 G +, position between patients and the control group. No significant differences were observed at the position of -1512 A/C. Also, no significant differences were seen between serum level of IL-13 cytokine and H. pylori disease (P >. 0.05).
Conclusion: Based on the results obtained, it seems that GA and GG genotypes of interleukin-13 gene at position 2044+ make people more susceptible to H. pylori infection.
Type of Study: Research |
Subject:
Special
Received: 2019/06/11 | Accepted: 2019/06/11 | Published: 2019/06/11
Received: 2019/06/11 | Accepted: 2019/06/11 | Published: 2019/06/11
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