Volume 14, Issue 1 (Winter 2024)
aumj 2024, 14(1): 1-16 |
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Mirzaii A, larijani K, Parkan N. Investigating the effects of silver nanoparticles synthesized by the green method on the expression of biofilm genes in Klebsiella pneumoniae strains. aumj 2024; 14 (1) :1-16
URL: http://aums.abzums.ac.ir/article-1-1782-en.html
URL: http://aums.abzums.ac.ir/article-1-1782-en.html
1- Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran , Amir.mirzaie@piau.ac.ir
2- Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract: (274 Views)
Introduction: "Klebsiella pneumonia" is an opportunistic human pathogen known for its ability to form biofilms, which contribute to multidrug resistance. This study investigates the green synthesis of silver nanoparticles and their effects on the expression of biofilm-related genes in "Klebsiella pneumonia" strains.
Material and method: In this cross-sectional descriptive study, 60 urine samples were collected and subjected to biochemical verification tests. Ethanolic extract of garlic ("Allium sativum") was prepared for the green synthesis of silver nanoparticles. Characterization of the synthesized nanoparticles was performed, and "Klebsiella pneumonia" biofilm formation was assessed by measuring color intensity associated with biofilm presence. The resistance pattern of the bacteria was determined using the disk diffusion method, and the minimum inhibitory concentration (MIC) of silver nanoparticles against biofilm-forming strains was evaluated using the microdilution method. Multiplex PCR was conducted to identify the genes "mrkD", "sugE", and "luxS", and the expression levels of these genes were analyzed using Real-Time PCR.
Result: Among the 60 samples, 36 were confirmed as "Klebsiella pneumonia", and all strains tested positive for biofilm formation. Most isolates were classified as multidrug resistant (MDR). The synthesized silver nanoparticles exhibited a spherical shape, black color, and an average size of 60 nm. They demonstrated antibacterial activity against all isolates, with MIC values ranging from 6.25 to 100 μg/ml. The highest frequency of the "luxS" gene was found in 30 isolates (83.33%), while "sugE" and "mrkD" were present in 23 isolates each (63.88%). Notably, the expression of "mrkD", "sugE", and "luxS" genes significantly decreased in cells treated with silver nanoparticles compared to the control group.
Conclusion: The study concludes that silver nanoparticles effectively inhibit biofilm formation and antibiotic resistance in "Klebsiella pneumonia" isolates, suggesting their potential utility in managing multidrug-resistant infections in hospitalized patients.
Material and method: In this cross-sectional descriptive study, 60 urine samples were collected and subjected to biochemical verification tests. Ethanolic extract of garlic ("Allium sativum") was prepared for the green synthesis of silver nanoparticles. Characterization of the synthesized nanoparticles was performed, and "Klebsiella pneumonia" biofilm formation was assessed by measuring color intensity associated with biofilm presence. The resistance pattern of the bacteria was determined using the disk diffusion method, and the minimum inhibitory concentration (MIC) of silver nanoparticles against biofilm-forming strains was evaluated using the microdilution method. Multiplex PCR was conducted to identify the genes "mrkD", "sugE", and "luxS", and the expression levels of these genes were analyzed using Real-Time PCR.
Result: Among the 60 samples, 36 were confirmed as "Klebsiella pneumonia", and all strains tested positive for biofilm formation. Most isolates were classified as multidrug resistant (MDR). The synthesized silver nanoparticles exhibited a spherical shape, black color, and an average size of 60 nm. They demonstrated antibacterial activity against all isolates, with MIC values ranging from 6.25 to 100 μg/ml. The highest frequency of the "luxS" gene was found in 30 isolates (83.33%), while "sugE" and "mrkD" were present in 23 isolates each (63.88%). Notably, the expression of "mrkD", "sugE", and "luxS" genes significantly decreased in cells treated with silver nanoparticles compared to the control group.
Conclusion: The study concludes that silver nanoparticles effectively inhibit biofilm formation and antibiotic resistance in "Klebsiella pneumonia" isolates, suggesting their potential utility in managing multidrug-resistant infections in hospitalized patients.
Keywords: Klebsiella pneumoniae, Biofilm, Silver Nanoparticle, Antimicrobial Resistance, Gene Expressionsion
Type of Study: Research |
Subject:
General
Received: 2024/02/23 | Accepted: 2024/05/18 | Published: 2024/12/30
Received: 2024/02/23 | Accepted: 2024/05/18 | Published: 2024/12/30
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