Volume 13, Issue 4 (Autumn 2024)                   aumj 2024, 13(4): 276-289 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fallah N, Babaei A, Momeni Z. Antibacterial effects of Trigonella foenum-graceum L. and Kellusia odoratissima Mozaff versus 0.2% chlorhexidine on Streptococcus mutans count: An in vitro study. aumj 2024; 13 (4) :276-289
URL: http://aums.abzums.ac.ir/article-1-1762-en.html
1- Student Research Committee, Alborz University of Medical Sciences, Karaj, Iran
2- Community Oral Health Department, School of Dentistry, Alborz University of Medical Sciences, Karaj, Iran
Abstract:   (545 Views)
Introduction
Tooth decay is a prevalent chronic disease worldwide; preventive care is essential for its management. Despite some side effects, Chlorhexidine mouthwash is the gold standard for controlling plaque and preventing caries. This study evaluates the antibacterial effects of "Trigonella foenum-graceum" L. and "Kellusia odoratissima" Mozaff extracts on "Streptococcus mutans" compared to 0.2% chlorhexidine mouthwash.
Materials & Methods
This laboratory study involved preparing herbal extracts of "Trigonella foenum-graceum" L. and "Kellusia odoratissima" Mozaff. The Well Diffusion Agar test measured the inhibition zone diameter at a concentration of 100,000 ppm for the extracts and chlorhexidine mouthwash, with chlorhexidine and distilled water serving as positive and negative controls, respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined for the extracts and mouthwash. Finally, the "Streptococcus mutans" count was assessed after exposure to the extracts and mouthwash. Data were analyzed using SPSS 26.0, applying the Kruskal-Wallis test for group comparisons and the Mann-Whitney U test for pairwise comparisons.
Results
The study reported three key findings. The inhibition zone diameters were 19 mm for chlorhexidine mouthwash, 0 mm for "Trigonella foenum-graceum" L., and 13 mm for "Kellusia odoratissima” Mozaff. MIC values were 6250 ppm for chlorhexidine, >100000 ppm for "Trigonella foenum-graceum", and 50000 ppm for "Kellusia odoratissima", with corresponding MBC values of 12500 ppm, >100000 ppm, and 100000 ppm. Significant differences in bacterial reduction percentages were noted after 30 minutes and 6 hours (both: p<0.001), with chlorhexidine showing the highest efficacy at 30 minutes, while "Kellusia odoratissima" Mozaff had the highest efficacy at 6 hours.
Conclusion
This study demonstrated that "Kellusia odoratissima" Mozaff extract has antibacterial effects comparable to chlorhexidine, while "Trigonella foenum-graceum" L. showed limited antibacterial activity against "Streptococcus mutans".
Full-Text [PDF 1526 kb]   (172 Downloads)    
Type of Study: Research | Subject: General
Received: 2023/12/07 | Accepted: 2024/02/18 | Published: 2024/11/30

References
1. Khoshnevisan M, Ghasemianpour M, Samadzadeh H, Baez R. Oral health status and healthcare system in IR Iran. J Contemp Med Sci. 2018;4(3):107-8.
2. Ezati F, Bakhshi E, Ezati E, Salimi N. Investigate of Tooth Decay and Its Relationship with Knowledge, Attitude and Performance (Kap) in High School Students in Islamabad-E-Gharb City. J Arak Uni Med Sci 2023;26(2):450-463. [DOI:10.32598/jams.25.3.6882.1]
3. Ryan KJ, Ray CG. Med Microbiol. McGraw Hill. 2004;4:370.
4. Nourizadeh Tehrani P. The Cost of All Types of Dental Services Presented by Farman Farmaeian Comprehensive Health Services Center at Tehran University of Medical Sciences using Activity-Based Costing Method in 1397. Hakim Res J. 2019;22(4):319-328.
5. Marthaler TM, Steiner M, Menghini G, Bandi A. Caries prevalence in Switzerland. Int Dent J. 1994;44(4 Suppl 1):393-401.
6. Babadi, F, Mohammadtaghvaei, N, Delnavaz S. Comparison of the effects of Jaftex and Persica mouthwashes on salivary pH and buffer capacity in the students and patients referred to the dentistry clinic of Sina hospital in Ahvaz. Jundishapur Sci Me J. 2019;18(1):13-21.
7. Nahvi M, Zarei E, Marzban S, Jahanmehr N. Utilization of dental services and its out-of-pocket payments: a study in dental clinics of Ramsar. J Mashhad Dent School 2017;41:171-182.
8. Neamatollahi A, Nekokah F. Comparison of the Antimicrobial Activity of ethanol Extract of Celery (Kelussia odoratissima) with common antibiotics used in Rainbow trout farms. J Aquac Res Dev. 2018;12(2):27-36.
9. Zajkani E, Abdi Z, Zeighami H. Evaluating the effect of 0. 05% fluoride and chlorhexidine 0. 2% mouthwashes in comparison to nanosil D1+ F mouthwash on Streptococcus mutans, in vitro. 2022;19(1)28-36. Nahvi M, Zarei E, Marzban S, Jahanmehr N. Utilization of dental services and its out-of-pocket payments: a study in dental clinics of Ramsar. J Mashhad Dent School 2017;41:171-182. [DOI:10.52547/jrds.19.1.28.]
10. Dini M. Scientific name of medicinal plants used in traditional medicine. Forest and Rangeland Research Institute Publication, Iran. 2006:299-300.
11. Al-Timimi LA. Antibacterial and anticancer activities of fenugreek seed extract. Asian Pacific journal of cancer prevention: APJCP. 2019;20(12):3771. [DOI:10.31557/APJCP.2019.20.12.3771. PMid:31870120 PMCid:PMC7173369]
12. Mozaffarian V. Two new genera of Iranian Umbelliferae. Botanicheskii Zhurnal (St Petersburg). 2003;2:88-94.
13. Ghasemi Pirbalouti A, Shah Wali A, Saghaee S, Azizi S, Hamedi B, Shahgholian L. Effect of chicory extract (cichorium intybus L.) and cultivars of Bakhtiari essense (Kleussia ordratassima Mizaff) on removal of organophosphoric toxin poisoning in rat. Herbal Medicine. 2011;2:31-36.
14. Salimi MA, Ebrahimi A, Asadieh ZS, Dehkordi SS. Essential oil composition of Kelussia odoratissima Mozaff. Iranian J Medicinal Aromatic Plants. 2010;26(2):147-156.
15. Rayesi M, Mohammadpour M, Feridouni M, Zare M, Heydari H. Investigating the bactericidal and bacteriostatic effect of hydroalcoholic extract of mint plant and sodium chloride salt on Streptococcus mutans bacteria. Res Sci J Ardabil Univ Med Sci Health Serv. 2015; 22(4)
16. Heidari Sureshjani M, Tabatabaei Yazdi F, Mortazavi A, Shahidi F. Comparison of the inhibitory and antibacterial effect of aqueous and ethanolic extract of Kelussia odoratissima on some pathogenic bacteria. Rafsanjan Uni Med Sci J. 2015;13(9):775-84.
17. Abiri R, Majnooni M, Malek Khattabi P, Adibi H. Study of antimicrobial effects of Trigonella Foenum hydro-alcoholic extract on different bacterial strains. Med Lab J. 2009;3(1).
18. Alwan AM, Jassim IM, Jasim GM. Study of antibacterial activities of seeds extract of fenugreek (trigonella foenum-graecum). Diyala J Med. 2017;13(1):62-67. [DOI:10.26505/DJM.13013360416]
19. Dharajiya D, Jasani H, Khatrani T, Kapuria M, Pachchigar K, Patel P. Evaluation of antibacterial and antifungal activity of fenugreek (Trigonella foenum-graecum) extracts. Int J Pharmacy and Pharmaceutical Sci. 2016;8(4):212-217.
20. Khotimah H, Wahyu Indriati D, Sundari AS. Screening in vitro antimicrobial activity of celery (Apium Graveolens) against Staphylococcus sp. Mal J Med Health Sci. 2020;16(Supp16):72-77.
21. Salman BN, Sallah S, Abdi F, Salahi S, Rostamizadeh K, Shabestari SB. The Comparison of Antimicrobial Effect of Nigella sativa Nanoparticle and Chlorhexidine Emulsion on the Most Common Dental Cariogenicic Bacteria. Med J Islam Repub Iran. 2021;35.
22. Mahmoudi R, Kosari M, Zare P, Barati S. Kelussia odoratissima essential oil: biochemical analysis and antibacterial properties against pathogenic and probiotic bacteria. J. Agroaliment. Processes Technol. 2014;20(1):109-5.
23. Babaei AH, Motamedifar M, Khalifat S, Mohammadi A, Zamani KH, Motamedifar A. In vitro study of antibacterial property and cytotoxic effects of aqueous, ethanolic, methanolic, and hydroalcoholic extracts of fenugreek seed. P J M H S 2018;12(2):906- 910.
24. Khanra R. Antimicrobial activity of ethanolic extract of Tigonella Foenum-Graecum Linn. Int Res J Pharm. 2010;1(1):181-183.
25. Al-Khalifa KS, AlSheikh R, Al-Hariri MT, El-Sayyad H, Alqurashi MS, Ali S, Bugshan AS. Evaluation of the Antimicrobial Effect of Thymoquinone against Different Dental Pathogens: An In Vitro Study. Molecules. 2021;26(21):6451. [DOI:10.3390/molecules26216451. PMid:34770860 PMCid:PMC8588385]
26. Swadas M, Dave B, Vyas SM, Shah N. Evaluation and comparison of the antibacterial activity against Streptococcus mutans of grape seed extract at different concentrations with chlorhexidine gluconate: An in vitro study. Int J Clin Pediatr Dent. 2016;9(3):181. [DOI:10.5005/jp-journals-10005-1360. PMid:27843246]

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Alborz University Medical Journal

Designed & Developed by : Yektaweb