Volume 12, Issue 1 (11-2022)
aumj 2022, 12(1): 77-84 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
khosravi F, Mohamadynejad P, moghanibashi M. Association of VNTR Polymorphism of NLRP3 Gene With the Risk of Gastric Cancer. aumj 2022; 12 (1) :77-84
URL: http://aums.abzums.ac.ir/article-1-1626-en.html
URL: http://aums.abzums.ac.ir/article-1-1626-en.html
1- Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
2- Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3- Department of Genetic, Faculty of Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
2- Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3- Department of Genetic, Faculty of Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Abstract: (1067 Views)
Background and aim: Gastric cancer is one of the most common cancers in the world, including Iran, which has a high mortality rate. One of the effective factors in the development of gastric cancer is the increase in inflammation caused by the increase in the function of the cryopyrin protein, which is encoded by the NLRP3 gene in humans. Increased expression of this gene leads to the production of IL-1β and IL-18 cytokines and increases inflammation. Considering the important role of NLRP3 gene, in this study, the role of VNTR polymorphism in intron 4 of NLRP3 gene in susceptibility to gastric cancer was investigated.
Materials and Methods: In this study, VNTR polymorphism of NLRP3 gene was genotyped in 100 patients with gastric cancer and 88 healthy individuals by PCR technique. The obtained data were analyzed using SPSS ν21 software and logistic regression test.
Results: In this study, 15 different genotypes and 7 alleles were identified in the VNTR polymorphism of NLRP3 gene, of which 12 replicate alleles and 12.12 genotypes were the most frequence. Also, allele with 14 repeat were identified for the first time. Analysis of the results showed that genotype 12.12 increase the risk of gastric cancer compared with other genotypes (p value = 0.01, CI = 0.14 - 0.76, OR = 0.32).
Conclusion: It seems that genotype 12.12 in the intron 4 of NLRP3 gene increased the risk of gastric cancer.
Materials and Methods: In this study, VNTR polymorphism of NLRP3 gene was genotyped in 100 patients with gastric cancer and 88 healthy individuals by PCR technique. The obtained data were analyzed using SPSS ν21 software and logistic regression test.
Results: In this study, 15 different genotypes and 7 alleles were identified in the VNTR polymorphism of NLRP3 gene, of which 12 replicate alleles and 12.12 genotypes were the most frequence. Also, allele with 14 repeat were identified for the first time. Analysis of the results showed that genotype 12.12 increase the risk of gastric cancer compared with other genotypes (p value = 0.01, CI = 0.14 - 0.76, OR = 0.32).
Conclusion: It seems that genotype 12.12 in the intron 4 of NLRP3 gene increased the risk of gastric cancer.
Type of Study: Research |
Subject:
Special
Received: 2022/10/24 | Accepted: 2022/11/01 | Published: 2022/11/01
Received: 2022/10/24 | Accepted: 2022/11/01 | Published: 2022/11/01
References
1. Malekzadeh R.Gastric Cancer in Iran: Epidemology and Risk Factors. Arch Iran Med. 2009; 12(6):576-583.
2. Inoue M, Tsugane S. Epidemiology of gastric cancer in Japan. Postgrad Med J. 2005; 81 (957): 419-24. [DOI:10.1136/pgmj.2004.029330]
3. Lichtenstein P, Holm NV, Verkasalo PK, Iliadou A, Kaprio J, Koskenvuo M, et al. Environmental and heritable factors in the causation of cancer-analyses of cohorts of twins from Sweden, Denmark, and Finland. N Engl J Med 2000; 343. [DOI:10.1056/NEJM200007133430201]
4. Zendehdel K,Marzban M,Nahvijou A,Jafari N,Six-fold Difference in the stomatch Mortality Rate between Northern and Southern Iran
5. Kwon SJ. Evaluation of the 7th UICC TNM Staging System of Gastric Cancer. J Gastric Cancer 2011; 11 (2): 78-85. [DOI:10.5230/jgc.2011.11.2.78]
6. kim BC, Kim WY,SNP@Promoter:a database of human SNPS Within the putative promoter regions. BMC Bioinformatics.2008.12-57
7. Örd T, Puurand T, Örd D, Annilo T, Möls M, Remm M, et al. A human-specific VNTR in the TRIB3 promoter causes gene expression variation between individuals. PLoS genetics. 2020;16(8):e1008981. [DOI:10.1371/journal.pgen.1008981]
8. Ghavidel M, Mansury D, Nourian K, Ghazvini K. The most common spoligotype of Mycobacterium bovis isolated in the world and the recommended loci for VNTR typing; A systematic review. Microbial pathogenesis 2018 ;118:310-5. [DOI:10.1016/j.micpath.2018.03.036]
9. Hoffman HM, Mueller JL, Broide DH, Wanderer AA, Kolodner RD. Mutation of a new gene encoding a putative pyrin-like protein causes familial cold autoinflammatory syndrome and Muckle-Wells syndrome. Nat Genet 2001; 29:301-5. [DOI:10.1038/ng756]
10. Mathews RJ, Sprakes MB, McDermott MF. NOD-like receptors and inflammation. Arthritis Res Ther 2009; 10(6):228. [DOI:10.1186/ar2525]
11. Chi-Jung Chung, Bo-Ying Bao, Polymorphism of nucleotide binding domain-like receptor protein 3 (NLRP3) increases susceptibility of total urinary arsenic to renal cell carcinoma. 2020 Apr 20; 10(1):6640. [DOI:10.1038/s41598-020-63469-8]
12. Toshinori Omi, Maki Kumada, An intronic variable number of tandem repeat polymorphisms of the cold-induced autoinflammatory syndrome 1 (CIAS1) gene modifies gene expression and is associated with essential hypertension. 2006 Dec;14(12):1295-305. [DOI:10.1038/sj.ejhg.5201698]
13. Allen I.C., TeKippe E., Woodford R.M.T., Uronis J.M., Holl E.K, Rogers A.B, et al. The NLRP3 inflammasome functions as a negative regulator of tumorigenesis during colitis-associated cancer. J Exp Med. 2010; 207(5):1045-56. [DOI:10.1084/jem.20100050]
14. Moossavi M., Parsamanesh N., Bahrami A., Atkin S.L., Sahebkar A. Role of the NLRP3 inflammasome in cancer. Mol Cancer 2018; 17(1):158. [DOI:10.1186/s12943-018-0900-3]
15. Lee, H.E., Lee, J.Y., Yang, G. et al. Inhibition of NLRP3 inflammasome in tumor microenvironment leads to suppression of metastatic potential of cancer cells. Sci Rep 2019; 9:12277. [DOI:10.1038/s41598-019-48794-x]
16. Li L, Hong Z. IL-1β/NF-kb signaling promotes colorectal cancer cell growth through miR-181a/PTEN axis. Arch Biochem Biophys. 2016; 604:20-6. [DOI:10.1016/j.abb.2016.06.001]
17. S Li 1, X Liang 1, L Ma 1, L Shen 1, T Li 1, L Zheng 1, et al.MiR-22 sustains NLRP3 expression and attenuates H. pylori-induced gastric carcinogenesis. Oncogene 2018; 37(7):884-896. [DOI:10.1038/onc.2017.381]
18. M Jaeger, A Carvalho , Association of a variable number tandem repeat in the NLRP3 gene in women with susceptibility to RVVC. 2016; 35(5):797-801. [DOI:10.1007/s10096-016-2600-5]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
