Volume 10, Issue 4 (Autumn 2021)
aumj 2021, 10(4): 475-482 |
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Ahmadi Nejad S, Amini K. Molecular Identification of Integron and Sulfonamide Resistance Genes in the Proteus Mirabilisis Isolated from Urine Samples by Multiplex-PCR and Their Antibiotic Resistance Profile. aumj 2021; 10 (4) :475-482
URL: http://aums.abzums.ac.ir/article-1-1432-en.html
URL: http://aums.abzums.ac.ir/article-1-1432-en.html
1- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Associated Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran ,dr_kumarss_amini@yahoo.com
2- Associated Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran ,
Abstract: (1583 Views)
Introduction: Proteus mirabilis is a common pathogen responsible for complicated urinary tract infections (UTIs) that sometimes causes bacteremia. The aim of the study was, Molecular identification of virulence genes at the same time intI, intII and sul in Proteus mirabilisis from urine Multiplex-PCR and Swarming Test.
Methods: In this cross-sectional study, 292 urine samples were collected. Antimicrobial susceptibility test was performed by disk diffusion test according to the clinical laboratory sard institute test (CLSI) on the Muller Hinton agar. Then, multiplex-PCR was achieved for determination of intI, intII and sul genes in the strains by specific oligonucleotides primers.
Results: Of 292 urine specimens, 60 P. mirabilis were obtained by standard microbiological and biochemical tests. The antimicrobial susceptibility result showed that the highest susceptibility rate were related to amikacine (100%) and gentamicin (98.3%). so, 10% of strains were resistance to ciprofloxacin. Distribution of these genes showed that 81.2% and 25% isolates were carring intII and sul1, respectively.
Discussion and Conclusion: Considering the role of integron in the development of resistance factors and the frequency of antibiotic resistance in Proteus mirabilis strains, paying attention to proper management in the use of antibiotics and taking necessary measures in compliance with health standards can be effective in preventing the spread of resistance.
Methods: In this cross-sectional study, 292 urine samples were collected. Antimicrobial susceptibility test was performed by disk diffusion test according to the clinical laboratory sard institute test (CLSI) on the Muller Hinton agar. Then, multiplex-PCR was achieved for determination of intI, intII and sul genes in the strains by specific oligonucleotides primers.
Results: Of 292 urine specimens, 60 P. mirabilis were obtained by standard microbiological and biochemical tests. The antimicrobial susceptibility result showed that the highest susceptibility rate were related to amikacine (100%) and gentamicin (98.3%). so, 10% of strains were resistance to ciprofloxacin. Distribution of these genes showed that 81.2% and 25% isolates were carring intII and sul1, respectively.
Discussion and Conclusion: Considering the role of integron in the development of resistance factors and the frequency of antibiotic resistance in Proteus mirabilis strains, paying attention to proper management in the use of antibiotics and taking necessary measures in compliance with health standards can be effective in preventing the spread of resistance.
Type of Study: Research |
Subject:
Special
Received: 2021/11/26 | Accepted: 2021/10/02 | Published: 2021/10/02
Received: 2021/11/26 | Accepted: 2021/10/02 | Published: 2021/10/02
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