Volume 10, Issue 3 (Summer 2021)
aumj 2021, 10(3): 317-328 |
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Amini K, Shojaei sadi B, Estabraghi E. Antibiotic Resistance, Biofilm and Forms of L. Staphylococcal Coagulase Positive and Negative Strains Isolated from Clinical Specimens by E-test and ELISA. aumj 2021; 10 (3) :317-328
URL: http://aums.abzums.ac.ir/article-1-1366-en.html
URL: http://aums.abzums.ac.ir/article-1-1366-en.html
1- Associated Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Asistant Professor, Department of Microbiology, School of Basic Sciences, Arak Branch, Islamic Azad University, Arak, Iran ,shojaee_sadi@yahoo.com
3- Faculty Member, Department of Veterinary Medicine, Shahrbabak Branch, Islamic Azad University, Shahrbabak, Iran
2- Asistant Professor, Department of Microbiology, School of Basic Sciences, Arak Branch, Islamic Azad University, Arak, Iran ,
3- Faculty Member, Department of Veterinary Medicine, Shahrbabak Branch, Islamic Azad University, Shahrbabak, Iran
Abstract: (1575 Views)
Introduction: Staphylococcus aureus is a micrococcal, gram positive and positive catalase family with oxidative metabolism and fermentation metabolism. Staphylococci are a large group of skin inhabitants, mammals are mammals. In some of these bacteria, the peptidoglycan layer has completely disappeared, and some still have small portions of it. The purpose of this study was to investigate antibiotic resistance, biofilm and forms of L. Coagulase positive and negative staphylococci isolated from clinical specimens by E-test and ELISA.
Methods: A total of 437 clinical specimens were collected from the Tehran Mehr Hospital and other treatment centers during 6 months and transferred to the laboratory. The specimens were first transferred to the Blood agar environment and then from colonies on specific environments for final diagnosis. The growth of bacteria in the four media was also evaluated by BHI Agar, BHI broth, LMP Agar and LPM broth. Biofilm measurements were performed by light absorption and using a well plate. In the E.test test, antimicrobial resistance such as Kirby-Boer test was used from the Muller Hinton Agar medium.
Findings: The highest resistance and antibiotic susceptibility were penicillin and gentamicin, amikacin, respectively. The results of the E-TEST test based on the halo diameter showed the highest resistance to erythromycin with 1 mm and the highest sensitivity to ciprofloxacin with 2 mm.
Discussion and Conclusion: The results of MIC determination on Staphylococcus aureus strains isolated by penicillin microdilution showed more resistance to ciprofloxacin. Consequently, L-forms, bacterial protoplasts and Spheroplasts are resistant to peptidoglycan synthesis, such as penicillins, cephalosporins and glycopeptide antibiotics (vancomycin and tiopelanin), due to the lack of peptidoglycans.
Methods: A total of 437 clinical specimens were collected from the Tehran Mehr Hospital and other treatment centers during 6 months and transferred to the laboratory. The specimens were first transferred to the Blood agar environment and then from colonies on specific environments for final diagnosis. The growth of bacteria in the four media was also evaluated by BHI Agar, BHI broth, LMP Agar and LPM broth. Biofilm measurements were performed by light absorption and using a well plate. In the E.test test, antimicrobial resistance such as Kirby-Boer test was used from the Muller Hinton Agar medium.
Findings: The highest resistance and antibiotic susceptibility were penicillin and gentamicin, amikacin, respectively. The results of the E-TEST test based on the halo diameter showed the highest resistance to erythromycin with 1 mm and the highest sensitivity to ciprofloxacin with 2 mm.
Discussion and Conclusion: The results of MIC determination on Staphylococcus aureus strains isolated by penicillin microdilution showed more resistance to ciprofloxacin. Consequently, L-forms, bacterial protoplasts and Spheroplasts are resistant to peptidoglycan synthesis, such as penicillins, cephalosporins and glycopeptide antibiotics (vancomycin and tiopelanin), due to the lack of peptidoglycans.
Type of Study: Research |
Subject:
Special
Received: 2021/08/27 | Accepted: 2021/09/01 | Published: 2021/09/01
Received: 2021/08/27 | Accepted: 2021/09/01 | Published: 2021/09/01
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