Volume 10, Issue 2 (Spring 2021)
aumj 2021, 10(2): 153-164 |
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Sahraeian S, Rajabpour H, Edalatmanesh M A. The Effect of Sodium Butyrate on Hippocampal Cell Damage and Apoptic Neurons Density in Cerebral Hypoxic-Ischemia Model. aumj 2021; 10 (2) :153-164
URL: http://aums.abzums.ac.ir/article-1-1330-en.html
URL: http://aums.abzums.ac.ir/article-1-1330-en.html
1- Department of Biology, College of Sciences, Shiraz
2- Branch, Islamic Azad University, Shiraz, Iran
3- Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran ,amin.edalatmanesh@gmail.com
2- Branch, Islamic Azad University, Shiraz, Iran
3- Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran ,
Abstract: (1512 Views)
Introduction and aim: Histone deacetylase inhibitors (HDACi) have neuroprotective effects on amelioration of cerebral ischemic injuries. This study was investigated the effects of sodium butyrate (SB) as a HDACi hippocampal cell damage and neuronal/dark neuronal density in a rat cerebral hypoxic ischemia (HI) model.
Materials and Methods: In this experimental study, 40 male Wistar rats (weight: 210±10 gr) were randomly divided into 4 groups; control, HI+NS, HI+SB150 and HI+SB300. To induce The HI model after complete occlusion of the left carotid artery, animals were placed in a hypoxic chamber (containing 7.6% oxygen) for one hour. Twenty-four hours after surgery, SB was injected intraperitoneally for one week at doses of 150 and 300 mg / kg body weight. Then, the hippocampus was histopathologically studied. Cell density and neuronal dark density in the CA1, CA2, CA3 and dentate gyrus subareas were assessed by stereological method.
Results: Cell damage with increased dark neurons was observed in the different subareas of hippocampus in HI+NS group compared with the control group (p˂0.05). However, treatment with SB dose-dependently reduced cell damage and increased neuronal density in all four different hippocampal subareas (p˂0.05).
Conclusion: SB with its neuroprotective effects suppresses hippocampal neuron damage and production of apoptotic neurons in the cerebral HI model.
Materials and Methods: In this experimental study, 40 male Wistar rats (weight: 210±10 gr) were randomly divided into 4 groups; control, HI+NS, HI+SB150 and HI+SB300. To induce The HI model after complete occlusion of the left carotid artery, animals were placed in a hypoxic chamber (containing 7.6% oxygen) for one hour. Twenty-four hours after surgery, SB was injected intraperitoneally for one week at doses of 150 and 300 mg / kg body weight. Then, the hippocampus was histopathologically studied. Cell density and neuronal dark density in the CA1, CA2, CA3 and dentate gyrus subareas were assessed by stereological method.
Results: Cell damage with increased dark neurons was observed in the different subareas of hippocampus in HI+NS group compared with the control group (p˂0.05). However, treatment with SB dose-dependently reduced cell damage and increased neuronal density in all four different hippocampal subareas (p˂0.05).
Conclusion: SB with its neuroprotective effects suppresses hippocampal neuron damage and production of apoptotic neurons in the cerebral HI model.
Type of Study: Research |
Subject:
Special
Received: 2021/07/11 | Accepted: 2021/05/31 | Published: 2021/05/31
Received: 2021/07/11 | Accepted: 2021/05/31 | Published: 2021/05/31
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