Volume 13, Issue 3 (Summer 2024)
aumj 2024, 13(3): 194-203 |
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Tavakoli-Far B, Hosseini M, Goudarzvand H, Choopani S, Goudarzvand M. GABA Signaling in Spatial Memory changes in Animal Model of Alzheimer’s disease. aumj 2024; 13 (3) :194-203
URL: http://aums.abzums.ac.ir/article-1-1804-en.html
URL: http://aums.abzums.ac.ir/article-1-1804-en.html
Bahareh Tavakoli-Far1 
, Marjan Hosseini2 , Hadi Goudarzvand3 , Samira Choopani4 , Mahdi Goudarzvand *5
, Marjan Hosseini2 , Hadi Goudarzvand3 , Samira Choopani4 , Mahdi Goudarzvand *5
1- Associate Professor of Pharmacology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran
2- Assistance Professor of Physiology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran
3- Master of Essential Chemistry, National Standard Organization, of Iran, Karaj, Iran
4- Master of Physiology, , Pasteur Institute, Tehran, Iran
5- Associate Professor of Physiology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran & Non-communicable Diseases Research Centre, Alborz University of Medical Sciences, Karaj, Iran
2- Assistance Professor of Physiology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran
3- Master of Essential Chemistry, National Standard Organization, of Iran, Karaj, Iran
4- Master of Physiology, , Pasteur Institute, Tehran, Iran
5- Associate Professor of Physiology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran & Non-communicable Diseases Research Centre, Alborz University of Medical Sciences, Karaj, Iran
Abstract: (614 Views)
Introduction: Alzheimer's disease is a progressive disease with mild memory loss that eventually leads to the loss of the ability to conduct conversation and react to the environment. Despite the proven role of the drugs used against the obvious causes of Alzheimer's disease, it still seems impossible to achieve an effective and permanent treatment for the treatment of Alzheimer's disease. So, investigating different angles of GABAergic system signaling can be introduced as a promising target for the development of anti-Alzheimer drugs.
Materials and Methods: To induce Alzheimer's disease, the β-amyloid toxin at a dose of 5µg/µl was injected bilaterally into the hippocampus of Wistar male rats, and the rats were then treated with bilateral injection of GABA-A receptor agonist (muscimol) and antagonist (bicuculline), at a dose of 100 ng/µl/side in the hippocampus for 4 days. To assess the spatial memory of the animals, the parameters of the distance traveled by the animals, latency time to reach the hidden platform, and velocity of the animals were analyzed in Morris water maze test.
Findings: The distance traveled and the latency time to reach the hidden platform increased in the Morris water test following the injection of beta-amyloid, and the muscimol increased the amount of this memory impairment. The injection of bicuculline could not significantly change the spatial memory of the animals.
Conclusion: The results of this study indicate the destructive effect of beta-amyloid toxin on spatial memory, as well as the destructive role of muscimol in memory consolidation and retrieval.
Materials and Methods: To induce Alzheimer's disease, the β-amyloid toxin at a dose of 5µg/µl was injected bilaterally into the hippocampus of Wistar male rats, and the rats were then treated with bilateral injection of GABA-A receptor agonist (muscimol) and antagonist (bicuculline), at a dose of 100 ng/µl/side in the hippocampus for 4 days. To assess the spatial memory of the animals, the parameters of the distance traveled by the animals, latency time to reach the hidden platform, and velocity of the animals were analyzed in Morris water maze test.
Findings: The distance traveled and the latency time to reach the hidden platform increased in the Morris water test following the injection of beta-amyloid, and the muscimol increased the amount of this memory impairment. The injection of bicuculline could not significantly change the spatial memory of the animals.
Conclusion: The results of this study indicate the destructive effect of beta-amyloid toxin on spatial memory, as well as the destructive role of muscimol in memory consolidation and retrieval.
Type of Study: Research |
Subject:
Special
Received: 2024/03/27 | Accepted: 2024/05/14 | Published: 2024/08/31
Received: 2024/03/27 | Accepted: 2024/05/14 | Published: 2024/08/31
References
1. Fahanik‐Babaei J, Sedighi M, Mehrabi S, et al. Preliminary report of Iranian Registry of Alzheimer's disease in Tehran province: A cross‐sectional study in Iran. Health Science Reports 2022;5(6): e952. [DOI:10.1002/hsr2.952]
2. Li Y, Sun H, Chen Z, et al. Implications of GABAergic neurotransmission in Alzheimer's disease. Frontiers in aging neuroscience 2016;8: 31. [DOI:10.3389/fnagi.2016.00031]
3. Carello-Collar G, Bellaver B, Ferreira PC, et al. The GABAergic system in Alzheimer's disease: a systematic review with meta-analysis. Molecular Psychiatry 2020;3:1-12.
4. Abbas G, Mahmood W, Kabir N. Recent progress on the role of GABAergic neurotransmission in the pathogenesis of Alzheimer's disease. Reviews in the Neurosciences 2016;27(4): 449-55. [DOI:10.1515/revneuro-2015-0062]
5. Záborszky L, Cullinan WE, Braun A. Afferents to basal forebrain cholinergic projection neurons: an update. The basal forebrain: Anatomy to function 1991: 43-100. [DOI:10.1007/978-1-4757-0145-6_2]
6. Xu Y, Zhao M, Han Y, Zhang H. GABAergic inhibitory interneuron deficits in Alzheimer's disease: implications for treatment. Frontiers in neuroscience 2020;14:660. [DOI:10.3389/fnins.2020.00660]
7. Ghit A, Assal D, Al-Shami AS, Hussein DEE. GABAA receptors: structure, function, pharmacology, and related disorders. Journal of Genetic Engineering and Biotechnology 2021;19(1): 1-15. [DOI:10.1186/s43141-021-00224-0]
8. Frye CA, Qrareya A, Llaneza DC, Paris JJ. Central actions of 3α, 5α-THP involving NMDA and GABAA receptors regulate affective and sexual behavior of female rats. Frontiers in Behavioral Neuroscience 2020;14: 11. [DOI:10.3389/fnbeh.2020.00011]
9. Ohno M, Yamamoto T, Watanabe S. Intrahippocampal injections of benzodiazepine and muscimol impair working memory but not reference memory of rats in the three-panel runway task. European journal of pharmacology 1992;219(2): 245-51. [DOI:10.1016/0014-2999(92)90302-K]
10. Nunez J. Morris water maze experiment. JoVE (Journal of Visualized Experiments) 2008(19): e897. [DOI:10.3791/897-v]
11. Kolarova M, García-Sierra F, Bartos A, et al. Structure and pathology of tau protein in Alzheimer disease. International journal of Alzheimer's disease 2012;2012. [DOI:10.1155/2012/731526]
12. Morishima‐Kawashima M, Ihara Y. Alzheimer's disease: β‐Amyloid protein and tau. Journal of neuroscience research 2002;70(3): 392-401. [DOI:10.1002/jnr.10355]
13. Knopman DS, Amieva H, Petersen RC, et al. Alzheimer disease. Nature reviews Disease primers 2021;7(1): 33. [DOI:10.1038/s41572-021-00269-y]
14. Czapski GA, Strosznajder JB. Glutamate and GABA in microglia-neuron cross-talk in Alzheimer's disease. International Journal of Molecular Sciences 2021;22(21): 11677. [DOI:10.3390/ijms222111677]
15. Zhang W, Xiong B-R, Zhang L-Q, et al. The role of the GABAergic system in diseases of the central nervous system. Neuroscience 2021;470: 88-99. [DOI:10.1016/j.neuroscience.2021.06.037]
16. Burjanadze MA, Dashniani MG, Solomonia RO, et al. Age-related changes in medial septal cholinergic and GABAergic projection neurons and hippocampal neurotransmitter receptors: relationship with memory impairment. Experimental Brain Research 2022;240(5): 1589-604. [DOI:10.1007/s00221-022-06354-2]
17. Marshall GA, Fairbanks LA, Tekin S, et al. Early-onset Alzheimer's disease is associated with greater pathologic burden. Journal of geriatric psychiatry and neurology 2007;20(1): 29-33. [DOI:10.1177/0891988706297086]
18. Allen CN, Crawford IL. GABAergic agents on the medial septal nucleus affect hippocampal theta rhythm and acetylcholine utilization. Brain research 1984;322(2): 261-7. [DOI:10.1016/0006-8993(84)90116-1]
19. Conway ME. Alzheimer's disease: Targeting the glutamatergic system. Biogerontology 2020;21(3): 257-74. [DOI:10.1007/s10522-020-09860-4]
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