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:: Volume 12, Issue 3 (Summer 2023) ::
aumj 2023, 12(3): 271-282 Back to browse issues page
The Study of antimicrobial and anti-biofilm synergistic effects of ciprofloxacin and Morus nigra extract against Pseudomonas aeruginosa
Marzieh Aghaie1 , Leila Asadpour * 2
1- MSc, Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
2- Associate Professor, Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract:   (567 Views)
Background and Aim: Pseudomonas aeruginosa is a Gram - negative bacterium and one of the most important opportunity seeker pathogens in hospital infections that are typically obtained simultaneously. This study was carried out with the aim of examining the synergistic effects of the antibiotic ciprofloxacin and extracts of Morus nigra in the control of the Pseudomonas aeruginosa biofilm.

Materials and Methods: In this study, synergistic effects of ciprofloxacin antibiotic and Morus nigra extracts were used to inhibit the growth and biofilm formation of the 10 isolates of multi drug resistant P.  aeruginosa. Disc diffusion method has been used to determine antibiotic resistance. To examine the synergistic antimicrobial activities between Morus nigra extract and ciprofloxacin, Checker titration method was used to calculate the fractional inhibitory concentration (FIC). The effect of Morus nigra extract, ciprofloxacin antibiotics and the combination of both of them on the expression level of pslA gene was investigated by Real time PCR.

Results: Morus nigra extract showed antimicrobial effect against MDR P.  aeruginosa strains. The use of this extract in combination with ciprofloxacin had an additive effect on inhibition of growth of P. aeruginosa and at least 60% reduction in biofilm formation of test isolates was achieved (P˂0.05). Also, Morus nigra extract alone and in combination with ciprofloxacin antibiotic decreased the pslA gene expression levels.

Conclusion: The present results are promising in regard to the possibility of using Morus nigra extract to increase the effect of sinus for antibiotics infections. The use of this extract, or instead of antibiotics, may be a way of reducing the use of antibiotics, thus reducing the side effects and treatment costs of infectious diseases.
Keywords: Herbal extract, Morus nigra, Synergism, Biofilm, P. aeroginosa
Full-Text [PDF 1113 kb]   (339 Downloads)    
Type of Study: Research | Subject: Special
Received: 2022/11/22 | Accepted: 2023/08/24 | Published: 2023/09/01
References
1. Corehtash ZG, Khorshidi A, Firoozeh F, Akbari H, Aznaveh AM. Biofilm formation and virulence factors among Pseudomonas aeruginosa isolated from burn patients. Jundishapur journal of microbiology. 2015 Oct;8(10). [DOI:10.5812/jjm.22345]
2. Bogiel T, Depka D, Rzepka M, Kwiecińska-Piróg J, Gospodarek-Komkowska E. Prevalence of the genes associated with biofilm and toxins synthesis amongst the Pseudomonas aeruginosa clinical strains. Antibiotics. 2021 Feb 28;10(3):241. [DOI:10.3390/antibiotics10030241]
3. Shafiei M, Ali AA, Shahcheraghi F, Saboora A, Noghabi KA. Eradication of Pseudomonas aeruginosa Biofilms Using the Combination of n-butanolic Cyclamen coum Extract and Ciprofloxacin. Jundishapur journal of microbiology. 2014 Feb;7(2). [DOI:10.5812/jjm.14358]
4. Mohammadzadeh A, Sharifi A. Bacterial biofilm and its inhibition mechanisms relying on anti-biofilm properties of plant compounds. Experimental animal Biology. 2016 Aug 22;5(1):71-81.
5. Khameneh B, Iranshahy M, Soheili V, Fazly Bazzaz BS. Review on plant antimicrobials: a mechanistic viewpoint. Antimicrobial Resistance & Infection Control. 2019 Dec;8(1):1-28. [DOI:10.1186/s13756-019-0559-6]
6. Haroun MF, Al-Kayali RS. Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug-resistant Staphylococcus aureus and Klebsiella pneumoniae strains. Iranian Journal of Basic Medical Sciences. 2016 Nov;19(11):1193.
7. Özgen M, Serçe S, Kaya C. Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits. Scientia horticulturae. 2009 Feb 3;119(3):275-9. [DOI:10.1016/j.scienta.2008.08.007]
8. Soltan-Dallal M., Didar Z, Assessment the Antimicrobial Impacts of Manganese and Iron Doped Zinc Oxide Nanoparticles against Shigella Flexneri. Journal of Sabzevar University of Medical Sciences. 2019;26(5):575-584.
9. Javid Rad E, Keshavarzi F. Prevalence of phzM and pvdA virulence genes in pseudomonas aeruginosa isolated from Infectious disease ward patients. Scientific Journal of Kurdistan University of Medical Sciences. 2018 Feb 10;22(6):74-83.
10. Oliveira VD, Souza MT, Zanotto ED, Watanabe E, Coraça-Huber D. Biofilm Formation and Expression of Virulence Genes of Microorganisms Grown in Contact with a New Bioactive Glass. Pathogens. 2020 Nov;9(11):927. [DOI:10.3390/pathogens9110927]
11. Vaghry P, Aletaha M. Anti-biofilm effect of peppermint extract on Pseudomonas aeruginosa that isolated of hospitalized patients in Tabriz city. Journal of Neyshabur University of Medical Sciences. 2019;2(23):73-87.
12. Aminnezhad S, Kermanshahi RK, Ranjbar R. Effect of Althaea officinalis extract on growth and biofilm formation in Pseudomonas aeruginosa. Journal of Pure and Applied Microbiology. 2016;10(3):1857-63.
13. Foroughi A. A review on medicinal plants; An emphasis on antimicrobial effects. Veterinary Researches & Biological Products. 2022 Mar 21;35(1):2-17.
14. Khalid N, Fawad SA, Ahmed I. Antimicrobial activity, phytochemical profile and trace minerals of black mulberry (Morus nigra L.) fresh juice. Pak J Bot. 2011 Dec 1;43(6):91-6.
15. Shukla RK, Painuly D, Shukla A, Kumar V, Singh J, Porval A, et al. Physical evaluation, proximate analysis and antimicrobial activity of Morus nigra seeds. International Journal of Pharmacy and Pharmaceutical Sciences. 2014;7(1):191-8.
16. Mazimba O, Majinda R, Motlhanka D. Antioxidant and antibacterial constituents from Morus nigra. Pharmacy and Pharmacology. 2011;5(6):751-4. [DOI:10.5897/AJPP11.260]
17. Mahmoudi H, Zare Fahim N, Alikhani M Y, Shokoohizadeh L. Investigation of antimicrobial effect of Berberine on ciprofloxacin and imipenem resistance Acinetobacter baumannii Isolated from Hamadan Hospitals. Iran J Med Microbiol. 2020; 14 (1) :44-54. [DOI:10.30699/ijmm.14.1.44]
18. Silva DM, COSTA PA, Ribon AO, Purgato GA, Gaspar DM, Diaz MA. Plant extracts display synergism with different classes of antibiotics. Anais da Academia Brasileira de Ciências. 2019 May 13;91 (2). [DOI:10.1590/0001-3765201920180117]
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Aghaie M, Asadpour L. The Study of antimicrobial and anti-biofilm synergistic effects of ciprofloxacin and Morus nigra extract against Pseudomonas aeruginosa. aumj 2023; 12 (3) :271-282
URL: http://aums.abzums.ac.ir/article-1-1745-en.html
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Volume 12, Issue 3 (Summer 2023) Back to browse issues page
نشریه دانشگاه علوم پزشکی البرز Alborz University Medical Journal
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