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AWT IMAGE

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:: Volume 12, Issue 3 (Summer 2023) ::
aumj 2023, 12(3): 271-282 Back to browse issues page
The Study of antimicrobial and anti-biofilm synergistic effects of ciprofloxacin and Morus nigra extract against Pseudomonas aeruginosa
Marzieh Aghaie , Leila Asadpour
Associate Professor, Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract:   (367 Views)
Background and Aim: Pseudomonas aeruginosa is a Gram - negative bacterium and one of the most important opportunity seeker pathogens in hospital infections that are typically obtained simultaneously. This study was carried out with the aim of examining the synergistic effects of the antibiotic ciprofloxacin and extracts of Morus nigra in the control of the Pseudomonas aeruginosa biofilm.

Materials and Methods: In this study, synergistic effects of ciprofloxacin antibiotic and Morus nigra extracts were used to inhibit the growth and biofilm formation of the 10 isolates of multi drug resistant P.  aeruginosa. Disc diffusion method has been used to determine antibiotic resistance. To examine the synergistic antimicrobial activities between Morus nigra extract and ciprofloxacin, Checker titration method was used to calculate the fractional inhibitory concentration (FIC). The effect of Morus nigra extract, ciprofloxacin antibiotics and the combination of both of them on the expression level of pslA gene was investigated by Real time PCR.

Results: Morus nigra extract showed antimicrobial effect against MDR P.  aeruginosa strains. The use of this extract in combination with ciprofloxacin had an additive effect on inhibition of growth of P. aeruginosa and at least 60% reduction in biofilm formation of test isolates was achieved (P˂0.05). Also, Morus nigra extract alone and in combination with ciprofloxacin antibiotic decreased the pslA gene expression levels.

Conclusion: The present results are promising in regard to the possibility of using Morus nigra extract to increase the effect of sinus for antibiotics infections. The use of this extract, or instead of antibiotics, may be a way of reducing the use of antibiotics, thus reducing the side effects and treatment costs of infectious diseases.
Keywords: Herbal extract, Morus nigra, Synergism, Biofilm, P. aeroginosa
Full-Text [PDF 1113 kb]   (235 Downloads)    
Type of Study: Research | Subject: Special
Received: 2022/11/22 | Accepted: 2023/08/24 | Published: 2023/09/01
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Aghaie M, Asadpour L. The Study of antimicrobial and anti-biofilm synergistic effects of ciprofloxacin and Morus nigra extract against Pseudomonas aeruginosa. aumj 2023; 12 (3) :271-282
URL: http://aums.abzums.ac.ir/article-1-1745-en.html
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Volume 12, Issue 3 (Summer 2023) Back to browse issues page
نشریه دانشگاه علوم پزشکی البرز Alborz University Medical Journal
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