Volume 6, Issue 4 (11-2017)
aumj 2017, 6(4): 283-290 |
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Chaharmahali N, Akhavan Sepahi A, Awwadi M R. Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin. aumj 2017; 6 (4) :283-290
URL: http://aums.abzums.ac.ir/article-1-680-en.html
URL: http://aums.abzums.ac.ir/article-1-680-en.html
1- Department of Microbiology, School of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, School of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran ,Akhavansepahy@gmail.com
3- Department of Nanotechnology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, School of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran ,
3- Department of Nanotechnology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
Abstract: (4582 Views)
Background and Objectives: Vitamin B2 (riboflavin) is a water-soluble vitamin and yellow. The aim of this study is to identify strains of Bacillus isolated from soil do with the ability to produce riboflavin by PCR.
Materials and Methods: Soil samples was mild randomly during 2 consecutive days the air. Sampling was performed of areas far from the sun and from 3 to 10 cm soil depth. The genus Bacillus were identified to species level using standard methods. Also isolates were approved identified using the PCR method. To study the riboflavin production of synthetic culture media were used and HPLC techniques.
Result: PCR using gene 16SrRNA test results showed that 32 strains obtained from a strain of Bacillus subtilis and 10 were 2 strain of Bacillus megaterium. The isolated strains of Bacillus megaterium PTCC 1250 with standard strains were positive of riboflavin production in synthetic medium and HPLC.
Conclusions: Bacillus subtilis bacteria living in the soil among the most important role in the production of riboflavin and riboflavin-producing strains to confirm the use of molecular methods is necessary.
Materials and Methods: Soil samples was mild randomly during 2 consecutive days the air. Sampling was performed of areas far from the sun and from 3 to 10 cm soil depth. The genus Bacillus were identified to species level using standard methods. Also isolates were approved identified using the PCR method. To study the riboflavin production of synthetic culture media were used and HPLC techniques.
Result: PCR using gene 16SrRNA test results showed that 32 strains obtained from a strain of Bacillus subtilis and 10 were 2 strain of Bacillus megaterium. The isolated strains of Bacillus megaterium PTCC 1250 with standard strains were positive of riboflavin production in synthetic medium and HPLC.
Conclusions: Bacillus subtilis bacteria living in the soil among the most important role in the production of riboflavin and riboflavin-producing strains to confirm the use of molecular methods is necessary.
Type of Study: Research |
Subject:
General
Received: 2018/01/10 | Accepted: 2018/01/10 | Published: 2018/01/10
Received: 2018/01/10 | Accepted: 2018/01/10 | Published: 2018/01/10
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