Volume 13, Issue 4 (Autumn 2024)
aumj 2024, 13(4): 242-253 |
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Research code: 3205/60/د/1400
Ethics code: IR.ABZUMS.AEC.1400.001
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Tavakoli-Far B, Hosseini A, Fallah-Huseini H, Mohammdi M, Goudarzvand M. The Anti-diabetic Effect of the Mixture of Trigonella Foenum-graecum, Vaccinium Arctostaphylost, Silibum Marianum, dioica Urtica, Melissa Officinalis and Citrulluscolocynthis Extracts in the Diabetic Rat Animal Model and Evaluation of Possible Liver Complications.. aumj 2024; 13 (4) :242-253
URL: http://aums.abzums.ac.ir/article-1-1823-en.html
URL: http://aums.abzums.ac.ir/article-1-1823-en.html
Bahareh Tavakoli-Far1 
, Asieh Hosseini2 , Hasan Fallah-Huseini3 , Mahdi Mohammdi4 , Mahdi Goudarzvand *5
, Asieh Hosseini2 , Hasan Fallah-Huseini3 , Mahdi Mohammdi4 , Mahdi Goudarzvand *5
1- Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran & Department of Physiology - Pharmacology - Medical Physics, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
2- Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
4- Medical Student, Alborz University of Medical Sciences, Karaj, Iran
5- Non-communicable Diseases Research Centre, Alborz University of Medical Sciences, Karaj, Iran & Department of Physiology - Pharmacology - Medical Physics, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
2- Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
4- Medical Student, Alborz University of Medical Sciences, Karaj, Iran
5- Non-communicable Diseases Research Centre, Alborz University of Medical Sciences, Karaj, Iran & Department of Physiology - Pharmacology - Medical Physics, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
Abstract: (660 Views)
Background: Diabetes Mellitus is a metabolic disorder characterized by insulin deficiency or dysfunction. This study aimed to investigate the anti-diabetic effects of a mixture of extracts from "Trigonella foenum-graecum", "Vaccinium arctostaphylos", "Silybum marianum", "Urtica dioica", "Melissa officinalis", and "Citrullus colocynthis" in a diabetic rat model, as well as to evaluate potential liver complications associated with this extract.
Methods: In this experimental study, thirty male Wistar rats were rendered diabetic through intraperitoneal administration of alloxan (60 mg/kg). One week post-induction, diabetic rats were randomly assigned to five groups of six. The negative control group received distilled water via gavage, the positive control group received metformin (100 mg/kg), and three experimental groups received daily gavage of the plant extract mixture at doses of 150, 300, and 600 mg/kg, respectively. After 30 days of treatment, blood samples were collected from the tail to assess fasting blood sugar (FBS) and liver biomarkers. Subsequently, the rats were anesthetized with ketamine and xylazine, and liver tissue was prepared for histopathological evaluation using hematoxylin-eosin staining and light microscopy.
Results: The extract at doses of 300 and 600 mg/kg significantly reduced FBS and hpp2 levels. However, the 600 mg/kg dose resulted in a significant increase in liver enzymes and induced liver tissue damage.
Conclusion: The mixture of plant extracts demonstrated an anti-diabetic effect at a dosage of 300 mg/kg without toxic effects on the liver. These findings suggest potential therapeutic benefits of the extract in managing diabetes while highlighting the need for caution at higher dosages.
Methods: In this experimental study, thirty male Wistar rats were rendered diabetic through intraperitoneal administration of alloxan (60 mg/kg). One week post-induction, diabetic rats were randomly assigned to five groups of six. The negative control group received distilled water via gavage, the positive control group received metformin (100 mg/kg), and three experimental groups received daily gavage of the plant extract mixture at doses of 150, 300, and 600 mg/kg, respectively. After 30 days of treatment, blood samples were collected from the tail to assess fasting blood sugar (FBS) and liver biomarkers. Subsequently, the rats were anesthetized with ketamine and xylazine, and liver tissue was prepared for histopathological evaluation using hematoxylin-eosin staining and light microscopy.
Results: The extract at doses of 300 and 600 mg/kg significantly reduced FBS and hpp2 levels. However, the 600 mg/kg dose resulted in a significant increase in liver enzymes and induced liver tissue damage.
Conclusion: The mixture of plant extracts demonstrated an anti-diabetic effect at a dosage of 300 mg/kg without toxic effects on the liver. These findings suggest potential therapeutic benefits of the extract in managing diabetes while highlighting the need for caution at higher dosages.
Type of Study: Research |
Subject:
Special
Received: 2024/06/23 | Accepted: 2024/10/05 | Published: 2024/11/30
Received: 2024/06/23 | Accepted: 2024/10/05 | Published: 2024/11/30
References
1. Zimmet PZ, Magliano DJ, Herman WH, Shaw JE. Diabetes: a 21st century challenge. The lancet Diabetes & endocrinology 2014;2(1): 56-64. [DOI:10.1016/S2213-8587(13)70112-8. PMid:24622669]
2. Association AD. Diagnosis and classification of diabetes mellitus. Diabetes care 2014;37(Supplement_1): S81-S90. [DOI:10.2337/dc14-S081. PMid:24357215]
3. Baig AA, Benitez A, Quinn MT, Burnet DL. Family interventions to improve diabetes outcomes for adults. Annals of the New York Academy of Sciences 2015;1353(1): 89-112. [DOI:10.1111/nyas.12844. PMid:26250784 PMCid:PMC4624026]
4. Ghorbani A. Best herbs for managing diabetes: a review of clinical studies. Brazilian Journal of Pharmaceutical Sciences 2013;49: 413-22. [DOI:10.1590/S1984-82502013000300003]
5. Olaiya CO, Soetan KO. A review of the health benefits of fenugreek (Trigonella foenum-graecum L.): Nutritional, Biochemical and pharmaceutical perspectives. Am J Soc Issues Humanit 2014;4: 3-12.
6. Ajabnoor MA, Tilmisany AK. Effect of Trigonella foenum graceum on blood glucose levels in normal and alloxan-diabetic mice. Journal of ethnopharmacology 1988;22(1): 45-9. [DOI:10.1016/0378-8741(88)90229-2. PMid:3352284]
7. Ribes G, rard, Sauvaire Y, et al. Effects of fenugreek seeds on endocrine pancreatic secretions in dogs. Annals of nutrition and metabolism 1984;28(1): 37-43. [DOI:10.1159/000176780. PMid:6703649]
8. Abidov M, Ramazanov A, Jimenez Del Rio M, Chkhikvishvili I. Effect of Blueberin on fasting glucose, C-reactive protein and plasma aminotransferases, in female volunteers with diabetes type 2: double-blind, placebo controlled clinical study. Georgian Medical News 2006(141): 66-72.
9. Vargas-Mendoza N, Madrigal-Santillán E, Morales-González Á, et al. Hepatoprotective effect of silymarin. World journal of hepatology 2014;6(3): 144. [DOI:10.4254/wjh.v6.i3.144. PMid:24672644 PMCid:PMC3959115]
10. Huseini HF, Fakhrzadeh H, Larijani B, Samani AS. Review of anti-diabetic medicinal plant used in traditional medicine. 2006.
11. Lirussi F, Beccarello A, Zanette G, et al. Silybin-beta-cyclodextrin in the treatment of patients with diabetes mellitus and alcoholic liver disease. Efficacy study of a new preparation of an anti-oxidant agent. Diabetes, nutrition & metabolism 2002;15(4): 222-31.
12. Dar SA, Ganai FA, Yousuf AR, et al. Pharmacological and toxicological evaluation of Urtica dioica. Pharmaceutical Biology 2013;51(2): 170-80. [DOI:10.3109/13880209.2012.715172. PMid:23036051]
13. Hirano T, Homma M, Oka K. Effects of Stinging Nettle Root Extracts and Their Steroidal Components on the Na+, K+-ATPase of the Benign Prostatic Hyperplasia1. Planta medica 1994;60(01): 30-3. [DOI:10.1055/s-2006-959402. PMid:7510891]
14. Khodsooz S, Moshtaghian J, Eivani M. Antihyperglycemic and antihyperlipidemic effects of hydroalcoholic extract of Melissa officinalis (Lemon Balm) in alloxan-induced diabetic rats. 2016.
15. Weidner C, Wowro SJ, Freiwald A, et al. Lemon balm extract causes potent antihyperglycemic and antihyperlipidemic effects in insulin‐resistant obese mice. Molecular nutrition & food research 2014;58(4): 903-7. [DOI:10.1002/mnfr.201300477. PMid:24272914]
16. Al-Ghaithi F, El-Ridi MR, Adeghate E, Amiri MH. Biochemical effects of Citrullus colocynthis in normal and diabetic rats. Molecular and cellular biochemistry 2004;261: 143-9. [DOI:10.1023/B:MCBI.0000028749.63101.cc. PMid:15362497]
17. Daradka H, Almasad MM, WSh Q, et al. Hypolipidaemic effects of Citrullus colocynthis L. in rabbits. Pakistan journal of biological sciences: PJBS 2007;10(16): 2768-71. [DOI:10.3923/pjbs.2007.2768.2771. PMid:19070101]
18. Pudziuvelyte L, Jakštas V, Ivanauskas L, et al. Different extraction methods for phenolic and volatile compounds recovery from Elsholtzia ciliata fresh and dried herbal materials. Industrial crops and products 2018;120: 286-94. [DOI:10.1016/j.indcrop.2018.04.069]
19. Gunjal S, Shirolkar SV. An overview of process parameters and spray drying agents involved in spray drying of herbal extracts. Paid J 2020;13: 102-18.
20. Kianbakht S, Abasi B, Dabaghian FH. Anti-hyperglycemic effect of Vaccinium arctostaphylos in type 2 diabetic patients: A randomized controlled trial. Forschende Komplementärmedizin/Research in Complementary Medicine 2013;20(1): 17-22. [DOI:10.1159/000346607. PMid:23727759]
21. Moghadam FH, Vakili-Zarch B, Shafiee M, Mirjalili A. Fenugreek seed extract treats peripheral neuropathy in pyridoxine induced neuropathic mice. EXCLI journal 2013;12: 282.
22. Huseini HF, Darvishzadeh F, Heshmat R, et al. The clinical investigation of Citrullus colocynthis (L.) schrad fruit in treatment of Type II diabetic patients: a randomized, double blind, placebo‐controlled clinical trial. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 2009;23(8): 1186-9. [DOI:10.1002/ptr.2754. PMid:19170143]
23. Véniant MM, Komorowski R, Chen P, et al. Long-acting FGF21 has enhanced efficacy in diet-induced obese mice and in obese rhesus monkeys. Endocrinology 2012;153(9): 4192-203. [DOI:10.1210/en.2012-1211. PMid:22798348]
24. Scudamore CL, Busk N, Vowell K. A simplified necropsy technique for mice: making the most of unscheduled deaths. Laboratory animals 2014;48(4): 342-4. [DOI:10.1177/0023677214536555. PMid:24876091]
25. Semwal DK, Kumar A, Aswal S, et al. Protective and therapeutic effects of natural products against diabetes mellitus via regenerating pancreatic β‐cells and restoring their dysfunction. Phytotherapy Research 2021;35(3): 1218-29. [DOI:10.1002/ptr.6885. PMid:32987447]
26. Baquer NZ, Kumar P, Taha A, et al. Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues. Journal of biosciences 2011;36: 383-96. [DOI:10.1007/s12038-011-9042-0. PMid:21654091]
27. Feshani AM, Kouhsari SM, Mohammadi S. Vaccinium arctostaphylos, a common herbal medicine in Iran: molecular and biochemical study of its antidiabetic effects on alloxan-diabetic Wistar rats. Journal of ethnopharmacology 2011;133(1): 67-74. [DOI:10.1016/j.jep.2010.09.002. PMid:20850514]
28. Maghrani M, Zeggwagh N-A, Lemhadri A, et al. Study of the hypoglycaemic activity of Fraxinus excelsior and Silybum marianum in an animal model of type 1 diabetes mellitus. Journal of Ethnopharmacology 2004;91(2-3): 309-16. [DOI:10.1016/j.jep.2004.01.008. PMid:15120454]
29. Chung MJ, Cho S-Y, Bhuiyan MJH, et al. Anti-diabetic effects of lemon balm (Melissa officinalis) essential oil on glucose-and lipid-regulating enzymes in type 2 diabetic mice. British journal of nutrition 2010;104(2): 180-8. [DOI:10.1017/S0007114510001765. PMid:20487577]
30. Kumar S, Kumar D, Saroha K, et al. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharmaceutica 2008;58(2): 215-20. [DOI:10.2478/v10007-008-0008-1]
31. Kaviarasan S, Naik G, Gangabhagirathi R, et al. In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds. Food chemistry 2007;103(1): 31-7. [DOI:10.1016/j.foodchem.2006.05.064.]
32. Ziaee M, Khorrami A, Khalighi-Sigaroudi F, Momtaz S. Study of the protective effects of Citrullus colocynthis against toxic effects of glucose in PC-12 cell line.
33. Costa AF, Flor LS, Campos MR, et al. Burden of type 2 diabetes mellitus in Brazil. Cadernos de saude publica 2017;33: e00197915. [DOI:10.1590/0102-311x00197915]
34. Rashidi AA, Mirhashemi SM, Taghizadeh M, Sarkhail P. Iranian medicinal plants for diabetes mellitus: a systematic review. Pakistan journal of biological sciences: PJBS 2013;16(9): 401-11. [DOI:10.3923/pjbs.2013.401.411. PMid:24498803]
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