Volume 13, Issue 1 (Winter 2024)
aumj 2024, 13(1): 34-43 |
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Ghorban Fallah F, Habibollahi H, Ahmad Sharbafi M. Studying the change in transcription level of beta-catenin gene in PANC-1 pancreatic cancer cell line treated with clove extract. aumj 2024; 13 (1) :34-43
URL: http://aums.abzums.ac.ir/article-1-1674-en.html
URL: http://aums.abzums.ac.ir/article-1-1674-en.html
1- Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract: (624 Views)
Pancreatic cancer development is a complex biological process in which various genes are involved. The β-catenin/Wnt signaling pathway plays an important role in the development of pancreatic cancer. In this research, the effect of clove polyphenol extract on PANC-1 pancreatic cancer cell line and the changes in β-catenin gene expression were evaluated. PANC-1 pancreatic cancer cell line was purchased from the Royan center of Isfahan. After successive passages in order to perform MTT assay, the cells were cultured in 96-well culture plates and treated for 24 hours with different concentrations of clove plant extract, and the absorbance of the samples was determined at a wavelength of 570 nm. By interpreting the results of the MTT test, the IC50 value of clove extract was determined. Based on IC50, PANC1 cells were cultured under treatment with extract and without treatment (control), and then using the kit, RNA extraction and cDNA synthesis were done from the cells. Using specific primers for β-catenin gene and actin reference gene, real-time PCR was performed and then interpreted by 2-DDCT method. The compounds in clove extract were also determined by mass GC method. The results of MTT assay showed that the IC50 of clove extract in PANC1 cell line is in the range of 10 mg/ml and with increasing concentration of clove extract, cell survival is completely decreased. Examining the transcriptional change of β-catenin gene under the treatment of IC50 concentration of clove extract also showed that this extract is able to decrease the expression of this gene by 1.29 times at the level of transcription at a significant level of p<0.05, which due to the unconventional increase of β-catenin catenin in all types of cancer, reducing the expression of this gene under the treatment of clove extract can be desirable.
Type of Study: Research |
Subject:
Special
Received: 2023/05/14 | Accepted: 2023/10/29 | Published: 2024/02/29
Received: 2023/05/14 | Accepted: 2023/10/29 | Published: 2024/02/29
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