Volume 12, Issue 1 (11-2022)
aumj 2022, 12(1): 42-48 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rostami H, Firoozeh F, Zibaei M, Salahshoorifar I, Sobhani-Nasab A, Nilkbin V S. Survey of Two Methods Including Phenol-Chloroform and Application of Magnetic Nanoparticles for Genomic DNA Extraction of Staphylococcus aureus. aumj 2022; 12 (1) :42-48
URL: http://aums.abzums.ac.ir/article-1-1622-en.html
URL: http://aums.abzums.ac.ir/article-1-1622-en.html
Hadiseh Rostami1 
, Farzaneh Firoozeh *2 
, Mohammad Zibaei3 , Iman Salahshoorifar4 , Ali Sobhani-Nasab5 , Vajihe Sadat Nilkbin6
, Farzaneh Firoozeh *2 , Mohammad Zibaei3 , Iman Salahshoorifar4 , Ali Sobhani-Nasab5 , Vajihe Sadat Nilkbin6
1- MSc, Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
2- Associate Professor, Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Research Center for Herbal Medicine and Complementary Medicine, Alborz University of Medical Sciences, Karaj, Iran
3- Research Center for Herbal Medicine and Complementary Medicine, Alborz University of Medical Sciences, Karaj, Iran Professor, Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
4- Assistant Professor, Department of Genetic, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
5- Assistant Professor, Physiology Research Center, Basic Sciences Research Institute, Kashan University of Medical Sciences, Kashan, Iran
6- PhD, Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
2- Associate Professor, Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Research Center for Herbal Medicine and Complementary Medicine, Alborz University of Medical Sciences, Karaj, Iran
3- Research Center for Herbal Medicine and Complementary Medicine, Alborz University of Medical Sciences, Karaj, Iran Professor, Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
4- Assistant Professor, Department of Genetic, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
5- Assistant Professor, Physiology Research Center, Basic Sciences Research Institute, Kashan University of Medical Sciences, Kashan, Iran
6- PhD, Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
Abstract: (1348 Views)
Background and Aim: Isolation of genomic DNA from bacterial cells is one of the processes typically performed in most biological laboratories and there are different methods to do it .In this study, two methods including phenol-chloroform, and magnetic nanoparticles, were used to extract genomic DNA of Staphylococcus aureus.
Materials and Methods: In the present study, the standard strain of Staphylococcus aureus ATCC 25923 was used for extraction of genomic DNA by phenol-chloroform and magnetic nanoparticles methods. Nanodrop and electrophoresis on agarose gel were used to evaluate the quality and concentration of extracted DNA.
Results: The concentration of extracted DNA by phenol-chloroform and magnetic nanoparticle) SiO2/Fe3O4) methods were obtained 550.4 and 131.6 µg/ml respectively.
Conclusion: From the findings of this study it can be concluded that due to the thick wall of Staphylococcus aureus, genomic DNA extraction by magnetic nanoparticles (Fe3O4/SiO2) have acceptable concentration and purity for molecular processes such as PCR, and can be used as an alternative to other extraction methods.
Materials and Methods: In the present study, the standard strain of Staphylococcus aureus ATCC 25923 was used for extraction of genomic DNA by phenol-chloroform and magnetic nanoparticles methods. Nanodrop and electrophoresis on agarose gel were used to evaluate the quality and concentration of extracted DNA.
Results: The concentration of extracted DNA by phenol-chloroform and magnetic nanoparticle) SiO2/Fe3O4) methods were obtained 550.4 and 131.6 µg/ml respectively.
Conclusion: From the findings of this study it can be concluded that due to the thick wall of Staphylococcus aureus, genomic DNA extraction by magnetic nanoparticles (Fe3O4/SiO2) have acceptable concentration and purity for molecular processes such as PCR, and can be used as an alternative to other extraction methods.
Keywords: Staphylococcus aureus, DNA extraction, Magnetic nanoparticles, Phenol-chloroform, Bacterial genome
Type of Study: Research |
Subject:
Special
Received: 2022/10/24 | Accepted: 2022/11/01 | Published: 2022/11/01
Received: 2022/10/24 | Accepted: 2022/11/01 | Published: 2022/11/01
References
1. Gao X , Yao X, Zhang Z, Jia L. Rapid and sensitive detection of Staphylococcus aureus assisted by polydopamine modified magnetic nanoparticles. Talanta 2018;186:147-153. [DOI:10.1016/j.talanta.2018.04.046]
2. Holden M, Feil E, Lindsay J, et al. Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance. Proceedings of the National Academy of Sciences 2004;101(26):9786-9791. [DOI:10.1073/pnas.0402521101]
3. Sosa-Acosta JR, Silva JA, Fernaʹndez-Izquierdo L, et al. Iron oxide nanoparticles (IONPs) with potential applications in plasmid DNA isolation. Coll Surf A 2018;545(2018):167-178. [DOI:10.1016/j.colsurfa.2018.02.062]
4. Niemirowicz K, Markiewicz KH, Wilczewska AZ, et al. Magnetic nanoparticles as new diagnostic tools in medicine. Adv Med Sci 2012;57(2):196-207. [DOI:10.2478/v10039-012-0031-9]
5. Cheng HR, Jiang N. Extremely rapid extraction of DNA from bacteria and yeasts. Biotechnol Lett 2006;28(1):55- 59. [DOI:10.1007/s10529-005-4688-z]
6. Park D. Genomic DNA isolation from different biological materials. Methods Mol Biol 2007; 353:3-13. [DOI:10.1385/1-59745-229-7:3]
7. Tafvizi F,Osareh A. Comparison of DNA extraction methods from fixed tissue in formalin. J Comp pathobiol Iran 2015;12(3):1677-1682.
8. Intorasoot S, Srirung R, Intorasoot A, et al. Application of gelatin-coated magnetic particles for isolation of genomic DNA from bacterial cells. Anal Biochem 2009; 386(2):291-292. [DOI:10.1016/j.ab.2008.12.032]
9. Berensmeier S. Magnetic particles for the separation and purification of nucleic acids. Appl Microbiol Biotechnol 2006;73(3):495-504. [DOI:10.1007/s00253-006-0675-0]
10. Saiyed ZM, Ramchand CN, Telang SD. Isolation of genomic DNA using magnetic nanoparticles as a solidphase support. J Phys Condens Matter 2008;20(20):204153. [DOI:10.1088/0953-8984/20/20/204153]
11. Ketaki K, Dipak V, Deshmukh M. Isolation of genomic DNA from Escherichia coli K12 Strain by using iron oxide nanoparticles. J Pharm Biol Sci 2015;10(3):49-52.
12. McBain SC, Yiu HHP, EI Haj A, et al. Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection. J Mater Chem2007;17(24):2561-2565. [DOI:10.1039/b617402g]
13. Chen XW, Mao QX, Liu JW, et al. Isolation/separation of plasmid DNA using hemoglobin modified magnetic nanocomposites as solid-phase adsorbent. Talanta 2012;100:107-112. [DOI:10.1016/j.talanta.2012.07.095]
14. Maye MM, Nykypanchuk D, Van Der Lelie D, et al. A simple method for kinetic control of DNA induced nanoparticle assembly. J Am Chem Soc 2006;128(43):14020-14021. [DOI:10.1021/ja0654229]
15. Oliveira CF, Paim TG, Reiter KC, et al. Evaluation of four different DNA extraction methods in coagulase-negative staphylococci clinical isolates. Rev Inst Med Trop Sao Paulo 2014;56(1):29-33. [DOI:10.1590/S0036-46652014000100004]
16. Chen L, Zhang J. Bioconjugated magnetic nanoparticles for rapid capture of gram-positive bacteria. J Biosens Bioelectron 2012;S11:1-5. [DOI:10.4172/2155-6210.S11-005]
17. Rahnama H, Sattarzadeh A, Kazemi F, et al. Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction. Anal Biochem 2016;513:68-76. [DOI:10.1016/j.ab.2016.08.029]
18. Wecke J, Lahav M, Ginsburg I, et al. Cell wall degradation of Staphylococcus aureus by lysozyme. Arch Microbiol 1982;131(2):116-123. [DOI:10.1007/BF01053992]
19. Chakraborti S, Chatterjee T, Joshi P, et al. Langmuir 2010;26(5):3506-3513. [DOI:10.1021/la903118c]
20. Xu Z, Liu XW, Ma YS, et al. Interaction of nano-TiO 2 with lysozyme: insights into the enzyme toxicity of nanosized particles. Environ Sci Pollut Res Int 2010;17(3):798-806. [DOI:10.1007/s11356-009-0153-1]
21. Koc K, Alveroglu E. Adsorption and desorption studies of lysozyme by Fe3O4-polymer nanocomposite via fluorescence spectroscopy. J Mol Struct 2015;1089(2015):66-72. [DOI:10.1016/j.molstruc.2015.02.038]
22. Mardaneh J, Mohammadzadeh AR, Masomian Z. Polyethylene Glycol 200; a rapid and inexpensive method for DNA extraction from gram positive and gram negative bacteria. Q Horizon Med Sci 2015;21:7-11.
23. Mirnejad R, Babavalian H, Moosazadeh Moghadam M, et al. Rapid DNA extraction of bacterial genome using laundry detergents and assessment of the efficiency of DNA in downstream process using polymerase chain reaction. Afr J Biothecnol 2012;11(1):173-178. [DOI:10.5897/AJB11.2259]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
