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aumj 2024, 13(1): 26-33 |
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Azimi-Alamouty M, Habibi , M, Ebrahimi A, Jamalpoor Z. Investigating the effect of amniotic membrane solution on the behavior of human umbilical cord mesenchymal stem cells in an in vitro condition. aumj 2024; 13 (1) :26-33
URL: http://aums.abzums.ac.ir/article-1-1610-en.html
URL: http://aums.abzums.ac.ir/article-1-1610-en.html
1- Trauma Research Center, Aja University of Medical Sciences, Tehran, Iran
2- Iranian tissue bank and research center, Gene, Cell and Tissue Institute, Tehran University of medical sciences
3- Department of Tissue Engineering, School of Advanced Technologies in Medicine, Royan Institute, Tehran, Iran
4- Cytotech and Bioinformatics Research Group, Tehran, Iran
2- Iranian tissue bank and research center, Gene, Cell and Tissue Institute, Tehran University of medical sciences
3- Department of Tissue Engineering, School of Advanced Technologies in Medicine, Royan Institute, Tehran, Iran
4- Cytotech and Bioinformatics Research Group, Tehran, Iran
Abstract: (662 Views)
Introduction and objectives: Mesenchymal stem cells are a promising approach for the regeneration of damaged tissues. Engineered scaffolds loaded with cells is one of the methods of cell transplantation at the site of injury. However, viability of the cells loaded in the scaffold is still a challenge. Growth factors have a proven role in increasing the metabolic activity of cells. Amniotic membrane solution is a rich source of growth factor. The aim of this study was to determine the optimal concentration of amniotic membrane solution on the behavior of mesenchymal stem cells in in vitro.
Methods: Amniotic membrane solution was prepared by enzymatic method in predetermined concentrations. Mesenchymal stem cells loaded in hydrogel were treated with different concentrations. The viability, proliferation, and metabolic activity of the cells were evaluated.
Results: Cytotoxicity of amniotic membrane solution was measured by MTT method. Increasing the concentration from 0.1 to 1 mg/ml did not show any toxicity, and at the concentration of 1.5 mg/ml, a decrease in optical density (OD=0.58±0.012) was observed compared to the control (OD=0.39±0.014). The metabolic activity of the cell loaded in the hydrogel at a concentration of 1 mg/ml had a significant increase compared to the control group ( p 0.012) and the amount of DNA content in this group (19.6 ± 0.9 ng/matrix) confirmed it.
Conclusion: It is suggested that amniotic membrane solution as a rich source of growth factor with an optimal dose of 1mg/ml increases the viability and metabolic activity of the MSCs-loaded scaffold for cell therapy.
Methods: Amniotic membrane solution was prepared by enzymatic method in predetermined concentrations. Mesenchymal stem cells loaded in hydrogel were treated with different concentrations. The viability, proliferation, and metabolic activity of the cells were evaluated.
Results: Cytotoxicity of amniotic membrane solution was measured by MTT method. Increasing the concentration from 0.1 to 1 mg/ml did not show any toxicity, and at the concentration of 1.5 mg/ml, a decrease in optical density (OD=0.58±0.012) was observed compared to the control (OD=0.39±0.014). The metabolic activity of the cell loaded in the hydrogel at a concentration of 1 mg/ml had a significant increase compared to the control group ( p 0.012) and the amount of DNA content in this group (19.6 ± 0.9 ng/matrix) confirmed it.
Conclusion: It is suggested that amniotic membrane solution as a rich source of growth factor with an optimal dose of 1mg/ml increases the viability and metabolic activity of the MSCs-loaded scaffold for cell therapy.
Type of Study: Original |
Subject:
Special
Received: 2022/09/30 | Accepted: 2022/12/14 | Published: 2024/02/29
Received: 2022/09/30 | Accepted: 2022/12/14 | Published: 2024/02/29
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