Volume 10, Issue 1 (Winter 2021)
aumj 2021, 10(1): 89-104 |
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Mokhtari A, Zahraee Salehi T, Estabraghi E. Frequency of Exotoxin A, Exoenzyme, Alginate and PprI and PprL Virulence Genes in Animal and Human Pseudomonas Aeruginosa Isolates and Determination of Antibiotic Resistance Pattern. aumj 2021; 10 (1) :89-104
URL: http://aums.abzums.ac.ir/article-1-1254-en.html
URL: http://aums.abzums.ac.ir/article-1-1254-en.html
1- Technical Assistant of the Department of Microbiology, Atiyeh Veterinary Laboratory, Tamin Atiyeh Salamat Alborz Knowledge Foundation Company, Alborz, Iran , alm3370@gmail.com
2- Professor of Microbiology Department, Faculty of Vetrinary Medicine, University of Tehran, Tehran, Iran
3- Assistant Professor, Department of Veterinary, shahre babak Branch, Islamic Azad University, shahre babak, Iran
2- Professor of Microbiology Department, Faculty of Vetrinary Medicine, University of Tehran, Tehran, Iran
3- Assistant Professor, Department of Veterinary, shahre babak Branch, Islamic Azad University, shahre babak, Iran
Abstract: (1839 Views)
Background and Aims: Pseudomonas aeruginosa is the most important cause of various nosocomial infections and mastitis in dairy cattle and the development of antibiotic resistance. The aim of this study was to determine the antibiotic resistance of Pseudomonas aeruginosa and the presence of virulence genes in human and animal samples.
Materials and Methods: In this study, 102 human and animal strains of Pseudomonas aeruginosa were studied. Multiplex PCR for virulence factors was used to detect specific genes. Disk diffusion and E-test were performed according to CLSI method with different antibiotics.
Results: The results of antibiogram of human isolates of Pseudomonas aeruginosa showed the highest resistance to ampicillin and cefepime antibiotics (100%), and the highest resistance in animal isolates to ampicillin (100%). The prevalence of oprI gene was detected in 100% of human isolates and all samples were negative for alg-D gene. Unlike human samples, all animal samples were negative for exoenzyme S and only exoenzyme Y was detected in 15 isolates (25%). Exoenzyme T was the most abundant in human isolates. 100% of the samples were positive for this exoenzyme.
Conclusion: High prevalence of virulence genes between human and animal isolates and attention to the function of these genes makes it necessary to identify the effect on animal and human tissues. Due to the high level of antibiotic resistance in animal and human strains, proper use and size of antibiotics should be observed.
Materials and Methods: In this study, 102 human and animal strains of Pseudomonas aeruginosa were studied. Multiplex PCR for virulence factors was used to detect specific genes. Disk diffusion and E-test were performed according to CLSI method with different antibiotics.
Results: The results of antibiogram of human isolates of Pseudomonas aeruginosa showed the highest resistance to ampicillin and cefepime antibiotics (100%), and the highest resistance in animal isolates to ampicillin (100%). The prevalence of oprI gene was detected in 100% of human isolates and all samples were negative for alg-D gene. Unlike human samples, all animal samples were negative for exoenzyme S and only exoenzyme Y was detected in 15 isolates (25%). Exoenzyme T was the most abundant in human isolates. 100% of the samples were positive for this exoenzyme.
Conclusion: High prevalence of virulence genes between human and animal isolates and attention to the function of these genes makes it necessary to identify the effect on animal and human tissues. Due to the high level of antibiotic resistance in animal and human strains, proper use and size of antibiotics should be observed.
Type of Study: Research |
Subject:
Special
Received: 2021/01/30 | Accepted: 2021/01/29 | Published: 2021/01/29
Received: 2021/01/30 | Accepted: 2021/01/29 | Published: 2021/01/29
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