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:: Volume 6, Issue 4 (11-2017) ::
aumj 2017, 6(4): 283-290 Back to browse issues page
Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin
Niloofar Chaharmahali, Abass Akhavan Sepahi , Mohammad Reza Awwadi
Department of Microbiology, School of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran , Akhavansepahy@gmail.com
Abstract:   (1111 Views)
Background and Objectives: Vitamin B2 (riboflavin) is a water-soluble vitamin and yellow. The aim of this study is to identify ‎strains of Bacillus isolated from soil do with the ability to produce riboflavin by PCR.
Materials and Methods: Soil samples was mild randomly during 2 consecutive days the air. Sampling was performed of areas far from the sun ‎and from 3 to 10 cm soil depth. The genus Bacillus were identified to species level ‎using standard methods. Also isolates were approved identified using the PCR method. To study the ‎riboflavin production of synthetic culture media were used and HPLC techniques.
Result: PCR using gene 16SrRNA test results showed that 32 strains obtained from a strain of Bacillus subtilis ‎and 10 were 2 strain of Bacillus megaterium. The isolated strains of Bacillus megaterium PTCC 1250 with ‎standard strains were positive of riboflavin production in synthetic medium and HPLC.
Conclusions: Bacillus subtilis bacteria living in the soil among the most important role in the production of ‎riboflavin and riboflavin-producing strains to confirm the use of molecular methods is necessary.
Keywords: Bacillus species, Riboflavin, 16SrRNA
Full-Text [PDF 359 kb]   (355 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/01/10 | Accepted: 2018/01/10 | Published: 2018/01/10
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Chaharmahali N, Akhavan Sepahi A, Awwadi M R. Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin. aumj. 2017; 6 (4) :283-290
URL: http://aums.abzums.ac.ir/article-1-680-en.html

Volume 6, Issue 4 (11-2017) Back to browse issues page
نشریه دانشگاه علوم پزشکی البرز Alborz University Medical Journal
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