fa بررسی miRNAها و mRNA ها در سلولهای بنیادی مزانشیمی در بیماری های قلبی- عروقی تخصصي Special پژوهشی کاربردی Original <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> شیوع</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بیماری قلبی عروقی</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">افزایش</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">سن و افزابش جمعیت جهان در حال گسترش است<b>.</b> </span></span><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">تغییرات سلولی، مولکولی و فیزیولوژیکی در قلب و عروق منجر به </span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بیماریهای قلبی می شود.</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بررسی</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مسیرهای</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">پاتوژنز</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">دقیق</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مولکولی</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بیماری</span></span><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> قلب</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">،</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">تشخیص</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">زود</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">هنگام</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">درمان</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">موثر</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">برای بیماری را فراهم می کند.</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">شناسایی</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بیومارکرهای</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">حساس</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">تشخیصی برای</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">کاربردهای</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بالینی</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بسیار</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">ارزشمند</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">موثر</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">است</span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> مطالعه</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">حاضر</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">هدف</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">یافتن </span></span><span dir="LTR" lang="X-NONE" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">ها و اهداف </span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">انها</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">انجام</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">شد</span></span><span dir="LTR" style="font-size:16.0pt"><span style="font-family:BNazanin">.</span></span><span dir="LTR" style="font-size:16.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">روش کار:</span></span></b><b> </b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مجموعه</span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA </span></span><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">&nbsp;و اهداف</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> ژنی انها در بیماری از پایگاه</span></span> <span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">اطلاعاتی</span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">GEO</span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> و</span></span> &nbsp;<span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;miRDB</span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> &nbsp;با بررسی آماری </span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">p<0.01</span></span> &nbsp;<span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">logFC#1</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مشخص شدند</span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">.</span></span> <span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA </span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">&nbsp;های</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">دارای</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بیشترین</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">تغییر</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بیان در &nbsp;آنژیوژنز انتخاب شدند. آنالیز </span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">منحنی </span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">ROC</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> برای حساسیت و اختصاصیت</span></span> <span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> ها </span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نرم</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">افزار</span></span> <span dir="LTR" style="font-size:14.0pt"><span new="" roman="" style="font-family:" times="">XLSTAT</span></span> &nbsp;&nbsp;<span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نسخه 2016 </span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">انجام شد. </span></span><span lang="FA" style="font-size:16.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">یافته ها</span></span></b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">: نتایج آنالیز آماری</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نشان</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">داد</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">که</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">370</span></span> <span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA </span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">&nbsp;دارای</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">بیان متفاوت هستند. از این تعداد</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">180 </span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> &nbsp;افزایش بیان و 120 </span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span> &nbsp;<span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">کاهش بیان معنی دار</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">و بقیه بدون تغییر معنی دار بوده اند. </span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">تجزیه</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">تحلیل</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مسیرهای</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مولکولی نشان</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">داد</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> که تعدادی از این </span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> ها</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">&nbsp;فرایندهای مختلف&nbsp; مانند &nbsp;آنژیوژنز،</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">هیپوکسی، آپوپتوز نقش دارند. &nbsp;همچنین</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مشخص</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">شد</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">که </span></span><span dir="LTR" style="font-size:16.0pt"><span inherit="" style="font-family:">miR-665</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> ، </span></span>&nbsp;<span dir="LTR" style="font-size:16.0pt"><span inherit="" style="font-family:">miR-216a-5p</span></span><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">و </span></span>&nbsp;<span dir="LTR" style="font-size:16.0pt"><span inherit="" style="font-family:">miR-34a </span></span><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">&nbsp;در</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> آنژیوژنز اهمیت دارند. برای این </span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"> ها &nbsp;آنالیز</span></span><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" times="">ROC </span></span><span style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">&nbsp;سطح زیر منحنی </span></span><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">0.86</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">با اختصاصیت 0.89 و حساسیت 0.87 </span></span><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">تعیین</span></span> <span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">شد</span></span><span dir="LTR" style="font-size:16.0pt"><span style="font-family:BNazanin">.</span></span><span dir="LTR" style="font-size:16.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <b><span dir="RTL" lang="AR-YE" style="font-size:16.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:">نتیجه گیری:</span></span></span></b><span dir="RTL" lang="AR-YE" style="font-size:16.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"> شناسایی تغییرات </span></span></span><span style="font-size:16.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">miRNA</span></span></span><span dir="RTL" lang="AR-YE" style="font-size:16.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:">&nbsp; و</span></span></span><span dir="RTL" lang="AR-YE" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> اهداف ژنهای درگیر آنها می تواند اثرات درمانی سلولهای بنیادی را افزایش دهد و به عنوان روش موثر در درمان بیماری های قلبی-عروقی </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">در</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نظر</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">گرفته</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">شوند</span></span></span><span dir="RTL" lang="AR-YE" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">.</span></span></span> <b>&nbsp;</b> <div><span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">Introduction: </span></span></span></b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">The prevalence of cardiovascular disease is increasing with the age and the increase in the world&#39;s population. Cellular, molecular and physiological changes in the heart and blood vessels can improve heart diseases. Investigating the precise molecular pathogenesis pathways of heart disease can lead to early diagnosis and effective treatment for the disease. </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">Accurate </span></span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">diagnostic biomarkers are very valuable and effective for clinical applications. The present study was conducted to find miRNAs and their target genes.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">Methods: </span></span></span></b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">The miRNAs and their gene targets in the disease were determined from the GEO and miRDB databases by statistical analysis of p<0.01 and logFC#1. MiRNAs with the most expression changes in angiogenesis were selected. ROC curve analysis for sensitivity and specificity of miRNAs was performed in XLSTAT software version 2016. </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Ethics Code in Iran </span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">IR.UMSU.REC.1398. 327.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">Results: </span></span></span></b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">The results of statistical analysis showed that 370 miRNAs have different expression. Of these, 180 miRNAs increased in expression, 120 miRNAs decreased in expression, and the others showed no significant change. Analysis of molecular pathways revealed that some of these miRNAs are involved in various processes including angiogenesis, hypoxia, and apoptosis. It was also found that miR-665, miR-216a-5p and miR-34a are important in angiogenesis. For these miRNAs, the ROC analysis determined the area under the curve to be 0.86 with a specificity of 0.89 and a sensitivity of 0.87.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f">Conclusion:</span></span></span></b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f1f1f"> Identification of miRNA changes and the targets of involved genes can increase the therapeutic effects of stem cells and be considered an effective method in the treatment of cardiovascular diseases. </span></span></span></span></span></span></span><br> &nbsp;</div> <div dir="rtl"></div> بیماری قلبی- عروقی, سلولهای بنیادی مزانشیمی, miRNA, mRNA Cardiovascular disease, mesenchymal stem cells, miRNAs, mRNAs 94 102 http://aums.abzums.ac.ir/browse.php?a_code=A-10-622-8&slc_lang=fa&sid=1 Fatemeh Mansouri فاطمه منصوری mansouri1600@hotmail.com 100319475328460014934 100319475328460014934 Yes Department of Genetics and Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran گروه ژنتیک و ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران