fa بررسی ترکیبات آنتی اکسیدانی و فعالیت ضد باکتریایی عصاره برگ و دانه بارهنگ ( Plantago major) و اثر سمیت آن بر سلول‌های سرطانی ریه تخصصي Special پژوهشي Research <p dir="RTL" style="text-align:justify"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> گیاهان دارویی منابع مهمی از ترکیبات آنتی‌اکسیدان هستند که می&shy;توانند برای درمان عفونت&shy;های باکتریایی و بیماری&shy;های ناشی از رادیکال&shy;های آزاد مناسب باشند؛ بنابراین</span></span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">هدف این پژوهش بررسی ترکیبات آنتی&shy;اکسیدانی و ضدباکتریایی عصاره&shy; برگ و دانه گیاه بارهنگ و اثر مهاری آن بر</span></span></span> <span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">روی</span></span></span> <span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سلول&shy;های سرطانی ریه است. </span></span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">روش کار:</span></span></span></b><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> بدین منظور، </span></span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نمونه&shy;ها از عباس‌آباد جمع&shy;آوری و عصاره&shy;گیری به روش خیساندن انجام شد. میزان فنل، فلاونوئید و آنتوسیانین کل عصاره&shy;ها&shy; تعیین شد. همچنین جهت تعیین اثر ضد سرطانی از روش </span></span></span><span dir="LTR" lang="EN-US" style="font-size:13.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MTT</span></span></span> <span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و فعالیت باکتریایی از روش انتشار دیسک استفاده شد</span></span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته &shy;ها:</span></span></span></b> <span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بیشترین میزان فنل و فلاونوئید کل در عصاره برگ به ترتیب 183/0</span></span></span><span lang="FA" style="font-size:13.0pt"><span cambria="" style="font-family:"><span style="color:black">&plusmn;</span></span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">90/13 و 003/0</span></span></span><span lang="FA" style="font-size:13.0pt"><span cambria="" style="font-family:"><span style="color:black">&plusmn;</span></span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> 194/0 میلی&shy;گرم بر گرم وزن خشک گیاه بدست آمد. </span></span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:">همچنین خاصیت ضد سرطانی عصاره&shy;ها و وابسته به غلظت و زمان بود به طوری که بیشترین درصد مهار رشد سلول&shy;ها در غلظت 5000 میلی&shy;گرم بر میلی&shy;لیتر برای عصاره&shy;های برگ و دانه به ترتیب 74/ 98 و 54/99 درصد در مدت زمان 72 ساعت بدست آمد. </span></span><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:">بیشترین هاله عدم رشد مربوط به باکتری گرم مثبت <i>استافیلوکوس اورئوس</i> و در غلظت 1000 گرم بر میلی&shy;لیتر برای عصاره&shy;های برگ و دانه </span></span><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:">مشاهده شد</span></span><span lang="AR-SA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:">.</span></span> <span lang="FA" style="font-size:13.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه &shy;گیری:</span></span></span></b><span lang="FA" style="font-size:13.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> نتایج این مطالعه پیشنهاد می&shy; کند که عصاره&shy; گیاه بارهنگ می&shy;تواند به‌عنوان یک منبع قابل‌دسترس از ترکیبات آنتی&shy;اکسیدان خصوصاً فلاونوئیدها &shy;باشد و به علت داشتن خاصیت ضد سرطانی بالا می&shy;تواند به‌عنوان یک گیاه دارویی علیه سرطان ریه مورد پژوهش‌های وسیع&shy;تری قرار گیرد.</span></span></span></span></span></span></span></span></p> <pre style="text-align:justify"> <span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span lang="EN-US" style="border:none windowtext 1.0pt; font-size:12.0pt; padding:0cm"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Introduction: </span></span></span></b><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Medicinal plants are </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">valuable</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> sources of antioxidant compounds that can be </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">effective in</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> treating bacterial infections and diseases caused by </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">oxidative stress. This study aimed</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> to investigate the antioxidant and antibacterial </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">properties</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> of the leaf and seed extracts of</span></span></span>&nbsp;<span lang="EN-US" style="border:none windowtext 1.0pt; font-size:12.0pt; padding:0cm"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Plantago major</span></span></span>&nbsp;<span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">and </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">their</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> inhibitory </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">effects</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> on lung cancer cells.</span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span lang="EN-US" style="border:none windowtext 1.0pt; font-size:12.0pt; padding:0cm"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Methods: </span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Samples</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> were collected from Abbas Abad and extracted </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">using the</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> soaking method. Total phenol, flavonoid, and anthocyanin contents were </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">quantified. The anticancer potential</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> of the extracts</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> was assessed using</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> the MTT method</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">, while</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> antimicrobial activity was determined by </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">the </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">disk diffusion </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">method</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">. The study was approved by the Ethics Committee of the Islamic Azad University (IR.IAU.TON.REC. 1402.089).</span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span lang="EN-US" style="border:none windowtext 1.0pt; font-size:12.0pt; padding:0cm"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Results: </span></span></span></b><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">The </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">leaf extract showed the </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">highest total phenol </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">content (</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">13.90 &plusmn; 0.183 </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">mg/g DW) </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">and </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">flavonoid content (</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">0.194 &plusmn; 0.003 mg/g DW</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">). The </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">anticancer </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">activity</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> of the extracts was concentration</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">-</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> and time</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">-dependent, with</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> the highest cell growth </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">inhibition (98.74% for leaf extract and 99.54% for seed extract) observed at </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">5000 mg/ml </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">after 72 hours. The</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> leaf and seed extracts </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">also exhibited strong antibacterial activity against&nbsp;</span></span></span><span lang="EN-US" style="border:none windowtext 1.0pt; font-size:12.0pt; padding:0cm"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Staphylococcus aureus</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">, with the highest zone of inhibition (non-growth area) at </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">1000 </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">mg</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">/ml.</span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span lang="EN-US" style="border:none windowtext 1.0pt; font-size:12.0pt; padding:0cm"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Conclusion: </span></span></span></b><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">The results </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">indicate that&nbsp;</span></span></span><span lang="EN-US" style="border:none windowtext 1.0pt; font-size:12.0pt; padding:0cm"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">Plantago major</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">&nbsp;extract is a rich</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> source of antioxidant compounds, </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">particularly</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> flavonoids, and </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">possesses significant</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> anti-cancer properties</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">. It holds potential for further</span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414"> research as a medicinal plant </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">in the treatment of </span></span></span><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#131414">lung cancer.</span></span></span></span></span></span></span></span></span> </pre> بارهنگ, ترکیبات آنتی اکسیدان, سرطان ریه, فعالیت ضد باکتریایی Plantago major, Antioxidant, Antibacterial Activity, Lung Cancer, Plant Extracts 357 368 http://aums.abzums.ac.ir/browse.php?a_code=A-10-1644-1&slc_lang=fa&sid=1 Azar zamani آذر زمانی چپرپردی Azarzamani24@gmail.com 100319475328460014267 100319475328460014267 No Department of Microbiology, To.C., Islamic Azad University,Tonekabon, Iran گروه میکروبیولوژی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران Simin Arian سیمین آرین Simin.aryan@yahoo.com 100319475328460014268 100319475328460014268 No Department of Microbiology, To.C., Islamic Azad University,Tonekabon, Iran گروه میکروبیولوژی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران Zynab khazaei زینب خزائی کوهپر dz.khazaei@gmail.com 100319475328460014269 100319475328460014269 No Department of Cellular and Molecular Biology, To.C., Islamic Azad University,Tonekabon, Iran ، گروه سلولی و مولکولی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران Mahdieh Houshani مهدیه هوشنی mhoshani@yahoo.com 100319475328460014270 0009-0000-5244-1543 Yes Department of Plant Physiology, To.C., Islamic Azad University,Tonekabon, Iran گروه فیزیولوژی گیاهی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران