fa بررسی اثرعصاره گیاه کانابیس(حشیش) بر میزان بیان ژن‌ها‌ی Bax وBcl-2 در سلول های بافت چربی در موش های صحرایی نر بالغ تخصصي Special پژوهشي Research <span style="font-size:9pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span b="" lotus="" style="font-family:">زمینه</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span b="" lotus="" style="font-family:">و</span></span></b><b> </b><b><span b="" lang="FA" lotus="" style="font-family:">هدف</span></b><b><span lang="FA" style="border:none windowtext 1.0pt; font-size:14.0pt; padding:0cm"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">امروزه</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">چاقی</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">به</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">عنوان</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">یکی</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">از</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">اصلی</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">ترین</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">مشکلات</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">بهداشت</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">عمومی</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">درسراسر</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">جهان</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> مطرح </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">است.</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">با</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">توجه</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">به</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">افزایش</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">احتمال</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">ابتلا</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">به</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">سرطان</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">در</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">افراد</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">چاق</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">و</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">نقش</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> آپوپتوزیس در پیش گیری از بروز بیماری هایی مانند </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">سرطان</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> مطالعه حاضر با هدف بررسی اثر کانابیس بر بیان ژن های آپوپتوتیک </span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">B‌ax</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> <a name="_Hlk55748730">و</a></span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">Bcl-2</span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">در سلول های بافت چربی انجام گردید. </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:9pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">مواد و روش ها:</span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">در این مطالعه تجربی از 32 سر رت نر بالغ که به</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">گروه های کنترل (تحت تیمار با سالین) و 3 گروه تجربی تحت تیمار درون صفاقی به مدت یک،دو و سه هفته با عصاره کانابیس در دوز</span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">ng/kg</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> 100 تقسیم شدند استفاده گردید. در هر گروه 24 ساعت بعد از آخرین تزریق، بافت چربی از ناحیه شکم خارج و بیان ژن‌های</span></span></span></span>&nbsp;<span dir="LTR" style="background:#fafafa"><span style="color:black">bcl-2</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> و</span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">bax&nbsp;</span></span><span style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> با روش</span></span></span></span>&nbsp;<span dir="LTR" style="background:#fafafa"><span style="color:black">RT-PCR&nbsp;</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> مورد بررسی قرار گرفت. نتایج با استفاده از آزمون های </span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">ANOVA</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> و توکی آنالیز شدند و معناداری اختلاف داده‌ها در سطح 05/0</span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">P<</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> در نظر گرفته شد.</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span><br> <span style="font-size:9pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">نتابج</span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">: نتایج حاصل از آنالیز داده های این مطالعه نشان داد که کانابیس باعث افزایش بیان ژن آپوپتوتیک </span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">B‌ax</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> و کاهش بیان ژن آنتی آپوپتوتیک </span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">Bcl-2</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> در پایان هفته اول در سطح05/0</span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">P<</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> و در هفته های دوم و سوم در سطح01/0</span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black">P<</span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black"> نسبت به گروه کنترل می‌شود.</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:9pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجه گیری</span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">: نتایج این مطالعه نشان داد که عصاره گیاه کانابیس موجب کاهش بیان ژن آنتی آپوپتوتیک</span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black"> Bcl2 </span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">&nbsp;می‌گردد و کاهش بیان ژن مذکور باعث افزایش بیان ژن</span></span></span></span><span dir="LTR" style="background:#fafafa"><span style="color:black"> Bax </span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span b="" lotus="" style="font-family:"><span style="color:black">در سلول های بافت چربی می‌گردد.</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="background:#fafafa"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:10pt"><span style="line-height:12pt"><span new="" roman="" style="font-family:" times=""><strong><span style="border:none windowtext 1.0pt; font-size:9.0pt; padding:0cm"><span style="background:#f1f2f5"><span style="font-family:" tahoma=""><span style="color:black">Introduction:</span></span></span></span></strong><span style="color:black"> Today, obesity is one of the main public health problems worldwide due to the increased risk of cancer in obese people and the role of apoptosis in preventing diseases such as cancer. Therefore, the aim of this study was to investigate the effect of cannabis on the expression of Bax and Bcl-2 apoptotic genes in adipose tissue cells.</span><span b="" dir="RTL" lang="FA" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:12pt"><span new="" roman="" style="font-family:" times=""><strong><span style="border:none windowtext 1.0pt; font-size:9.0pt; padding:0cm"><span style="background:#f1f2f5"><span style="font-family:" tahoma=""><span style="color:black">Methods:</span></span></span></span></strong><span style="color:black"> In this experimental study, 32 adult male rats were divided into control groups (treated with saline) and 3 experimental groups treated intraperitoneally for one, two and three weeks with cannabis extract at a dose of 100 ng / kg. In each group, 24 hours after the last injection, adipose tissue was removed from the abdomen and the expression of bcl-2 and bax genes were examined by RT-PCR. </span><span b="" dir="RTL" lang="FA" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:12pt"><span new="" roman="" style="font-family:" times=""><strong><span style="border:none windowtext 1.0pt; font-size:9.0pt; padding:0cm"><span style="background:#f1f2f5"><span style="font-family:" tahoma=""><span style="color:black">Results:</span></span></span></span></strong><span style="color:black"> The results were analyzed using ANOVA and Tukey tests and the difference in data was considered significant at the level of P<0.05.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:12pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The results of data analysis of this study showed that cannabis increased the expression of Bax apoptotic gene and decreased the expression of anti-apoptotic Bcl-2 gene at the end of the first week at the level of P <0.05 and in the second and third weeks at the level of P <0.01 compared to The group is controlled.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:12pt"><span new="" roman="" style="font-family:" times=""><strong><span style="border:none windowtext 1.0pt; font-size:9.0pt; padding:0cm"><span style="background:#f1f2f5"><span style="font-family:" tahoma=""><span style="color:black">Conclusion:</span></span></span></span></strong>&nbsp;<span style="color:black"> The results of this study showed that cannabis extract reduces the expression of Bcl2 anti-apoptotic gene and decreases the expression of this gene increases the expression of Bax gene in adipose tissue cells.</span><span b="" dir="RTL" lang="FA" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:12pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span><br> &nbsp; کانابیس, سلول های چربی, Bax, Bcl-2, Cannabis, Fat cells, Bax, Bcl-2 325 338 http://aums.abzums.ac.ir/browse.php?a_code=A-10-472-4&slc_lang=fa&sid=1 Arman Kamali-Sarvestani آرمان کمالی سروستانی 10031947532846009632 10031947532846009632 No Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran. گروه زیست‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران SE Hosseini سیدابراهیم حسینی 10031947532846009633 10031947532846009633 Yes Department of Biology, Faculty of Science, Zand Institute of Higher Education, Shiraz, Iran. گروه آموزشی زیست شناسی ،دانشکده علوم، موسسه آموزش عالی زند شیراز، شیراز، ایران D Mehraban داوود مهربان 10031947532846009634 10031947532846009634 No Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran مرکز تحقیقات و فناوری سلول‌های بنیادی، دانشگاه علوم پزشکی شیراز، شیراز، ایران مرکز تحقیقات سوختگی و زخم ، دانشگاه علوم پزشکی شیراز، شیراز، ایران SS Hashemi ساره هاشمی 10031947532846009635 10031947532846009635 No Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran مرکز تحقیقات سوختگی و زخم ، دانشگاه علوم پزشکی شیراز، شیراز، ایران