Showing 4 results for Phenol
M. Noorisepehr, A.r. Yazdanbakhsh, H. Daraei,
Volume 1, Issue 1 (1-2012)
Abstract
Background: The removal of phenol by biosorbent has received increasing interest in recent years. In this study the potential of ostrich feathers was assessed for adsorption of phenol from aqueous solutions. Materials and Methods: In this research, feasibility of application ostrich feather and modified feather was studied at the laboratory scale using a synthetic phenol solution (1-50) mg/L and the impact of the important field parameters including pH, sorbents concentration, retention time and temperature were investigated. Then, the adsorption data were described with Langmuir and Freundlich adsorption isotherms and Excel software for analyzing the data was used. Result: The result obtained revealed that by increasing the dosage of the sorbents from 0.2 g to 0.7 g the adsorption increases. The adsorption of phenol decreases by the increase of the pH value of the solution. Also, it is observed that the contact time significantly affect the rate of phenol adsorption (83% for raw feathers and 73% for modified feather) from solution. Conclusion: The Results showed that efficiency of raw feather process is more than of modified feather process for phenol removal. The results showed that the ostrich feathers as an economic material and disposal solid waste from poultry processing plants can be used as an effective biosorbent for phenol.
1 H. Daraei, 2 F. Daraei,
Volume 2, Issue 4 (10-2013)
Abstract
Background & Objectives: Phenol is one of the hazardous pollutants in industrials effluent. This pollutant is very toxic to human and the environment. The purpose of this study was investigation of phenol adsorption capacity with egg shell waste from aqueous solution. Methods: The eggshells were dried at 70◦C and for 12 hr in oven and then shells were crushed. The surface characteristics and quantitative elemental information were studied with SEM and XRF analysis. Then the Freundlich and Longmuir adsorption isotherms and Kinetic models were applied to description of the experimental data. Results: The maximum adsorption took place in first 60min of reaction and at pH 9. With increasing the temperature the pollutant adsorption was decreased. Also absorption characteristics of this pollutant on eggshell accommodated with Freundlich isotherm (R2=0.996). Phenol removal kinetics were fitted with both pseudo-first order and pseudo-second order kinetic models. Conclusion: The presented results demonstrate clearly that eggshell is a potentially useful material to be used for the removal of phenol from aqueous solution and industrial wastewater.
Ala Amiri, Farzaneh Firoozeh, Mohammad Zibaei, Iman Salahshoorifar,
Volume 9, Issue 3 (6-2020)
Abstract
Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least time and cost. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is one of the novel methods of nucleic acid isolation. The aim of this study is to compare DNA extracted from Escherichia coli using two techniques including phenol-chloroform and magnetic nanoparticles.
Methods: In this study, Escherichia coli strain ATCC25922 was used to compare the two extraction methods. For genomic DNA extraction the phenol-chloroform methods as well as the technique of using bilayer SiO2/Fe3O4 magnetic nanoparticles were used. Both products were analyzed by nanodrop and following agarose gel electrophoresis.
Results: The results of optical density (OD) evaluation with nanodrop spectrophotometer showed that the concentration of extracted DNA with conventional phenol-chloroform method and magnetic nanoparticle extraction technique were 761.4 µg/ml and 531 µg/ml respectively.
Conclusion: According to the obtained results by comparing the optical absorption of the two products and comparative evaluation with control groups in magnetic nanoparticle extraction method, also by comparing different factors such as the amount of time required, costs, ease of use, it can be concluded that due to the time-consuming and dangerous nature of the phenol-chloroform method, the using magnetic nanoparticles is an appropriated method for extracting the genomic content.
Hadiseh Rostami , Farzaneh Firoozeh, Mohammad Zibaei, Iman Salahshoorifar, Ali Sobhani-Nasab, Vajihe Sadat Nilkbin ,
Volume 12, Issue 1 (11-2022)
Abstract
Background and Aim: Isolation of genomic DNA from bacterial cells is one of the processes typically performed in most biological laboratories and there are different methods to do it .In this study, two methods including phenol-chloroform, and magnetic nanoparticles, were used to extract genomic DNA of Staphylococcus aureus.
Materials and Methods: In the present study, the standard strain of Staphylococcus aureus ATCC 25923 was used for extraction of genomic DNA by phenol-chloroform and magnetic nanoparticles methods. Nanodrop and electrophoresis on agarose gel were used to evaluate the quality and concentration of extracted DNA.
Results: The concentration of extracted DNA by phenol-chloroform and magnetic nanoparticle) SiO2/Fe3O4) methods were obtained 550.4 and 131.6 µg/ml respectively.
Conclusion: From the findings of this study it can be concluded that due to the thick wall of Staphylococcus aureus, genomic DNA extraction by magnetic nanoparticles (Fe3O4/SiO2) have acceptable concentration and purity for molecular processes such as PCR, and can be used as an alternative to other extraction methods.
