A. Roshan, H. Nayebzadeh, M. Zibaei, H.r. Shokrani, M.j. Tarahi,
Volume 5, Issue 4 (10-2016)
Abstract
Background: Treatment of hydatidosis in humans, mainly surgery with the use of drugs is combined. During surgery, the risk of fluid leakage hydatid cyst that was content to adjacent tissues, resulting in recurrence of the disease. Ephedra is a plant that grows in various regions of Iran and used to treat as antibacterial and antifungal in certain diseases. The aim of this study was to in vitro evaluate the effects of different concentrations of aqueous extract of Ephedra major at different times.
Materials and Methods: Infected liver to hydatid cysts were collected from slaughterhouses. Protoscoleces viability was assessed by staining Eosin 1.0 percent. Protoscoleces activities of aqueous extract 1/0, 01/0, 001/0 mg/ml at 10, 20, 30 and 60 minutes were analyzed. Saline and saturated brine were used as positive and negative controls.
Results: The highest percentage of household protoscoleces dilution of 0.1 (13.58%) was observed (P= 0.001). Protoscoleces lowest scolicidal effect rate was found in the dilution 0.001 (2.47%)
Conclusions: The effect of aqueous extract of Ephedra major plumbing protoscoleces not significant. Therefore, it cannot be used as a cache protoscoleces strong and appropriate.
Mahdis Sharifian Zade, Azadeh Khalili, Mohammad Zibaei, Abolfazl Miahipour,
Volume 14, Issue 1 (12-2024)
Abstract
Background and Objective: Hydatid cysts are significant zoonotic diseases, with surgical intervention being the most effective treatment, particularly for large cysts located in critical areas such as the brain or heart. However, the use of chemical scolicidal agents to prevent protoscolex leakage during surgery has been limited due to their adverse side effects. Consequently, there is increasing interest in exploring new antimicrobial compounds, particularly from plants, as promising alternatives. This study aimed to investigate the lethal effects of cannabidiol, a well-known cannabinoid derived from the cannabis plant, on hydatid cyst protoscolexes.
Methods: Protoscoleces were extracted from the livers of animals infected with hydatid cysts and subsequently exposed to various concentrations of cannabidiol (1, 5, and 10 μg/ml) for 10, 30, 60, and 120 minutes. The viability of the protoscoleces was assessed using 0.1% eosin staining, with albendazole serving as a standard control for comparison.
Results: Cannabidiol exhibited significant scolicidal activity across all tested concentrations, demonstrating a direct relationship between concentration and effectiveness. While albendazole produced a scolicidal effect of 30.82 ± 0.5% after two hours, cannabidiol at concentrations of 5 and 10 μg/ml achieved scolicidal effects of 42.52 ± 0.2% and 51.04 ± 0.44%, respectively.
Conclusion: This study demonstrates that cannabidiol effectively induces significant destruction of protoscolexes, with efficacy positively correlated to concentration. These findings suggest that cannabidiol could be a valuable candidate for further research as a potential therapeutic agent in the management of hydatid cysts, warranting additional studies to explore its applicability in clinical settings.