Showing 2 results for Mohseni
K. Haratian, A. Mohseni Meybodi,
Volume 1, Issue 2 (3-2012)
Abstract
Background: Measles virus (MV) is a highly contagious agent which causes a major health problem in developing countries. We studied the effect of BERBERINE on the replication of an AIK-HDC strain of MV and its induced apoptosis in Vero cell lines. Methods: In this study, toxicity of BERBERINE on Vero cells was investigated first, resulted in determination of sub-lethal or non-toxic concentration zone of BERBERINE for cells. Next, anti-viral effect of BERBERINE at various time limits was evaluated and virus titer was determined at each stage either as 50% tissue culture infective dose TCID50 or by plaque assay method. Using specific anti-measles IgG, anti-viral effect of BERBERINE on MV replication cycle was evaluated through indirect immunofluorescence assay, meanwhile presence of viral RNA was investigated by RT-PCR and gel- electrophoresis. Results: According to the experiments, BERBERINE, at concentration of 50 μM, markedly inhibited the cytopathic effect (CPE) induced by MV. BERBERINE also significantly inhibited apoptosis induced by MV. BERBERINE either influences replication of MV genome, or may inhibit virion formation. Conclusion: These results suggest that the inhibition of CPE and apoptosis by BERBERINE induced by MV may be associated with the effect of BERBERINE on viral RNA genome. Therefore, it is suggested that MV infections can induce apoptosis through the activation of a common pathway that can be blocked by BERBERINE or some of its ingredients.
Sattar Jafari, Mehran Mohseni, Saeideh Mazloomzadeh, Saeed Rezaei,
Volume 10, Issue 2 (Spring 2021)
Abstract
Back ground: Gastric cancer is an important, prevalant and second most fatal cancer in the world. Previous studies show heavy metals have various roles in human health. Heavy metals are known to be associated with various cancers but their function in gastric adenocarcinoma is unclear. In this study we examined serum level of these metals in gastric adenocarcinoma patients compared to control group.
Method: In total, 114 subjects (54 controls and 60 cancer patients) were included in this study. We took blood samples from 60 known cases of gastric adenocarcinoma hospitalized in oncology Ward of Valiaasr Hospital and 53 none cancerous patients hospitalized in other wards of the Valiasr hospital, as control group. After sample preparation, serum concentration of Zinc, Cadmium, Lead and Copper were determined by polarography and the obtained data were analyzed by SPSS software.
Results: Serum level of Copper was significantly higher in patients with gastric adenocarcinoma compared to control group (P-Vlue= 0.04). No significant differences were found in serum level of zinc, lead and cadmium in gastric adenocarcinoma patients compared to control group (P-Value > 0.05), however the mean concentration of zinc and Lead were higher in cancerous patients.
Conclusion: Findings in this study appear to support that Copper overload may be risk factor of Gastric Adenocarcinoma but to find out the exact roles of theses heavy metals, more extended studies are needed. Recently, tendency to use copper containing dishes has been increased in our society, so necessary warnings should be given to the community as to the possible carcinogenicity of copper.
