:: Volume 8, Issue 3 (8-2019) ::
aumj 2019, 8(3): 272-280 Back to browse issues page
Identification of Oral Strains of Lactobacillus species in Patients with Chronic Periodontitis and Healthy Individuals
Enayat Kalantar , Arghavan Etebarian, Seyed Mahmood Amin Marashi, Koroush Kabir, Aboozar Moradi, Atefe Shamosi, Tahere Sheshpari
Department of Microbiology, School of medicine, Alborz University Medical Sciences, Karaj, Iran
Abstract:   (986 Views)
Background and aim: Chronic periodontitis has been defined as an infectious disease resulting in inflammation within the dental supporting tissue, progressive attachment loss and bone resorption. Nowadays, with increasing microbial resistance, bacterial therapy is an alternative to prevent inflammatory diseases of the oral cavity, which regulates oral microbiota and eliminating pathogenic bacteria. The aim of this study was to identify oral Lactobacillus species and in patients with periodontitis and healthy individuals.
Materials & Methods: Use of specific culture medium and restriction fragment-length polymorphism (RFLP) analysis was applied to characterize oral lactobacillus in oral cavity samples (saliva, tongue, gingival plaque) from 59 healthy subjects and 59 patients with chronic periodontitis. The 16srDNA genes of lactobacillus .spp in samples were amplified by PCR with universal primers. Sequencing performed to confirm the species.
Results: Out Of 354 examined isolates, 254 isolates were positive for Lactobacillus based on culture and PCR results. From obtained strain cutting patterns analysis of the oral lactobacillus community, were aggregated into ten different species. L.paracasei, L.fermentumin patient and L.casei, L.plantarum were the most predominant species in control groups. 
Conclusion: The frequency of Lactobacillus in patients with periodontitis was significantly reduced comparing to the control group, especially in gingival plaque samples. The difference in the frequency of isolated lactobacillus in the total sample of patients was 8% less than control subjects. There is increasing evidence that the use of existing lactobacillus strains can prevent chronic periodontitis. These probiotic candidates can be selected for the purpose of bacteriotherapy, dairy manufacture and pharmaceutical use.
Keywords: Chronic periodontitis disease, 16SrDNA RFLP-PCR, Oral strains lactobacillus .spp
Full-Text [PDF 377 kb]   (241 Downloads)    
Type of Study: Research | Subject: Special
Received: 2019/08/09 | Accepted: 2019/08/09 | Published: 2019/08/09



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Volume 8, Issue 3 (8-2019) Back to browse issues page