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Molecular identification of Neisseria gonorrhoeae and Toxoplasma gondii isolated from infertile women with vaginal swab samples by Multiplex-PCR
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Elnaz Faroughi   , Kumarss Amini  |
| Association Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , kumarss_amini@yahoo.com |
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Abstract: (1200 Views) |
Background: Toxoplasma gondii and Neisseria gonorrhoeae are the rare causes of sexually transmitted infections. The aim of this study was detection of N. gonorrhoeae and T. gondii in patients with vaginal infection using Multiplex PCR.
Materials and methods: This cross sectional study was conducted in 60 infertile female patients with symptomatic vaginal infection, referring to Tehran Emam Khomeini hospital. After completing a demographic questionnaire, sixty vaginal swabs were obtained from each participant using individual sterile swabs. DNA extraction was performed according to manufacture CinnaGen kit. The multiplex polymerase chain reaction (M-PCR) was conducted based on specific primers for N. gonorrhoeae and T. gondii.
Results: Our finding indicated the frequency of infection with N. gonorrhoeae and T. gondii was 6.6% and 10%, respectively. No coinfection with N. gonorrhoeae and T. gondii was detected.
Conclusion: The Multiplex PCR is a good results for detection of N. gonorrhoeae and T. gondii in vaginal infection. The results of this study showed a relatively low frequency of N. gonorrhoeae and T. gondii in Tehran. |
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| Keywords: Vaginal samples, Toxoplasma gondii, Neisseria gonorrhoeae, Multiplex-PCR |
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Full-Text [PDF 1349 kb]
(465 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2021/08/27 | Accepted: 2021/09/01 | Published: 2021/09/01
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