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:: Volume 10, Issue 3 (Summer 2021) ::
aumj 2021, 10(3): 317-328 Back to browse issues page
Antibiotic Resistance, Biofilm and Forms of L. Staphylococcal Coagulase Positive and Negative Strains Isolated from Clinical Specimens by E-test and ELISA
Kumarss Amini , Behrooz Shojaei sadi , Ehsan Estabraghi
Asistant Professor, Department of Microbiology, School of Basic Sciences, Arak Branch, Islamic Azad University, Arak, Iran , shojaee_sadi@yahoo.com
Abstract:   (1186 Views)
 Introduction: Staphylococcus aureus is a micrococcal, gram positive and positive catalase family with oxidative metabolism and fermentation metabolism. Staphylococci are a large group of skin inhabitants, mammals are mammals. In some of these bacteria, the peptidoglycan layer has completely disappeared, and some still have small portions of it. The purpose of this study was to investigate antibiotic resistance, biofilm and forms of L. Coagulase positive and negative staphylococci isolated from clinical specimens by E-test and ELISA.
Methods: A total of 437 clinical specimens were collected from the Tehran Mehr Hospital and other treatment centers during 6 months and transferred to the laboratory. The specimens were first transferred to the Blood agar environment and then from colonies on specific environments for final diagnosis. The growth of bacteria in the four media was also evaluated by BHI Agar, BHI broth, LMP Agar and LPM broth. Biofilm measurements were performed by light absorption and using a well plate. In the E.test test, antimicrobial resistance such as Kirby-Boer test was used from the Muller Hinton Agar medium.
Findings: The highest resistance and antibiotic susceptibility were penicillin and gentamicin, amikacin, respectively. The results of the E-TEST test based on the halo diameter showed the highest resistance to erythromycin with 1 mm and the highest sensitivity to ciprofloxacin with 2 mm.
Discussion and Conclusion: The results of MIC determination on Staphylococcus aureus strains isolated by penicillin microdilution showed more resistance to ciprofloxacin. Consequently, L-forms, bacterial protoplasts and Spheroplasts are resistant to peptidoglycan synthesis, such as penicillins, cephalosporins and glycopeptide antibiotics (vancomycin and tiopelanin), due to the lack of peptidoglycans.

Keywords: Staphylococcus aureus, Antibiogram, ELISA test, Biofilm
Full-Text [PDF 1512 kb]   (554 Downloads)    
Type of Study: Research | Subject: Special
Received: 2021/08/27 | Accepted: 2021/09/01 | Published: 2021/09/01
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Amini K, Shojaei sadi B, Estabraghi E. Antibiotic Resistance, Biofilm and Forms of L. Staphylococcal Coagulase Positive and Negative Strains Isolated from Clinical Specimens by E-test and ELISA. aumj 2021; 10 (3) :317-328
URL: http://aums.abzums.ac.ir/article-1-1366-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 10, Issue 3 (Summer 2021) Back to browse issues page
نشریه دانشگاه علوم پزشکی البرز Alborz University Medical Journal
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