fa نانولیپوزوم حاوی کوپلیمر پلی آنیلین-پلی اکریلیک: سنتز، مشخصه‌یابی و بررسی القای آپوپتوز در سلول های سرطانی پستان (MCF-7) تخصصي Special پژوهشي Research <span style="font-size:12pt"><span style="background:white"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:#202122"><strong><span lang="FA" style="border:none windowtext 1.0pt; font-size:14.0pt; padding:0in"><span style="background:white"><span b="" mitra="" style="font-family:"><span style="color:black">مقدمه</span></span></span></span></strong><strong><span lang="AR-SA" style="border:none windowtext 1.0pt; font-size:14.0pt; padding:0in"><span style="background:white"><span b="" mitra="" style="font-family:"><span style="color:black">:</span></span></span></span></strong><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> کوپلیمر پلی آنیلین-پلی اکریلیک نسل جدید از مواد با خاصیت ضد سرطانی هستند.</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> هدف از این مطالعه سنتز و مشخصه یابی نانولیپوزوم حاوی کوپلیمر پلی آنیلین-پلی اکریلیک و بررسی اثر مهارکنندگی آن روی رشد سلول های سرطانی پستان </span></span>MCF-7)<span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">) در مقایسه با سلولهای نرمال بود.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="background:white"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:#202122"><strong><span lang="AR-SA" style="border:none windowtext 1.0pt; font-size:14.0pt; padding:0in"><span style="background:white"><span b="" mitra="" style="font-family:"><span style="color:black">روش‌ کار:</span></span></span></span></strong><b> </b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه نخست سنتز و مشخصه یابی کوپلیمر پلی آنیلین-پلی اکریلیک و نانولیپوزوم های حاوی کوپلیمر پلی آنیلین-پلی اکریلیک انجام گردید. اثرات سلولی این ترکیبات روی سلول های سرطانی (</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">7-</span></span>MCF<span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">) و </span></span>HFF <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">(بعنوان سلول نرمال) بعد از مواجهه 48ساعته با تست </span></span>MTT<span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">فلوسایتومتری و </span></span>Realtime PCR<span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> ارزیابی شد. &nbsp;</span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="background:white"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:#202122"><strong><span lang="AR-SA" style="border:none windowtext 1.0pt; font-size:14.0pt; padding:0in"><span style="background:white"><span b="" mitra="" style="font-family:"><span style="color:black">یافته‌ها:</span></span></span></span></strong><b> </b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">نتایج تست </span></span>MTT<span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> نشان داد درصد مرگ سلول های سرطانی </span></span>MCF-7<span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp; و </span></span>HFF <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">بعد از مواجهه با کوپلیمر پلی آنیلین-پلی اکریلیک و نانولیپوزوم حاوی کوپلیمر پلی آنیلین-پلی اکریلیک بصورت وابسته به غلظت عمل کردند. نانولیپوزوم حاوی کوپلیمر پلی آنیلین-پلی اکریلیک نسبت به کوپلیمر پلی آنیلین-پلی اکریلیک&nbsp; بیشتر باعث مرگ سلول های سرطانی شدند (05/0</span></span>P<<span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">). </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">نتایج تست آپوپتوز نشان داد که کوپلیمر پلی آنیلین-پلی اکریلیک و نانولیپوزوم حاوی کوپلیمر پلی آنیلین-پلی اکریلیک توانستند بطور معنی داری باعث القای آپوپتوز و افزایش سلول های آپوپتوزی زودرس و دیررس در</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> سلول های سرطانی شوند</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">(05/0</span></span>P<<span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">افزایش بیان </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">ژن های کاسپاز 3 و 9 در سلول های</span></span> <span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">7-</span></span>MCF<span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> نیز فعال سازی مسیر آپوپتوز را تایید کرد. </span></span><b><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></b></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="background:white"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:#202122"><strong><span lang="AR-SA" style="border:none windowtext 1.0pt; font-size:14.0pt; padding:0in"><span style="background:white"><span b="" mitra="" style="font-family:"><span style="color:black">نتیجه‌گیری</span></span></span></span></strong><strong><span lang="AR-SA" style="border:none windowtext 1.0pt; font-size:14.0pt; padding:0in"><span style="background:white"><span calibri="" style="font-family:"><span style="color:black">: </span></span></span></span></strong><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">این مطالعه بما نشان داد که کوپلیمر پلی آنیلین-پلی اکریلیک توانایی القای آپوتوز در سلولهای سرطانی </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">7-</span></span>MCF &nbsp;<span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">را دارد و این قابلیت با نانولیپوزومه کردن آن تاحدی افزایش می یابد. </span></span><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="font-family:&quot;Times New Roman&quot;,serif"><strong>Introduction:</strong> Polyaniline&ndash;polyacrylic copolymers represent an emerging class of polymeric materials with promising anticancer properties. The development of nanocarrier-based delivery systems may further enhance their biological activity and improve their selective effects on malignant cells. The present study aimed to synthesize and characterize nanoliposomes containing polyaniline&ndash;polyacrylic copolymers and to investigate their cytotoxic and apoptotic effects on breast cancer cells (MCF-7) in comparison with normal human cells.</span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="font-family:&quot;Times New Roman&quot;,serif"><strong>Methods:</strong> In this experimental study, polyaniline&ndash;polyacrylic copolymers and nanoliposomal formulations containing the synthesized copolymers were initially prepared and characterized. The cellular effects of the free polyaniline&ndash;polyacrylic copolymer and its nanoliposomal formulation were subsequently evaluated in MCF-7 breast cancer cells and human foreskin fibroblast (HFF) cells as a normal cell model. Following 48 hours of exposure, cellular viability and cytotoxicity were assessed using the MTT assay. Apoptotic cell death and changes in the distribution of apoptotic cell populations were evaluated by flow cytometry. In addition, the expression levels of apoptosis-related genes, including caspase-3 and caspase-9, were investigated using real-time polymerase chain reaction (real-time PCR). The study was conducted under the ethical approval code IR.IAU.PS.REC.1403.564.</span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="font-family:&quot;Times New Roman&quot;,serif"><strong>Results:</strong> The MTT assay demonstrated that exposure to both the polyaniline&ndash;polyacrylic copolymer and its nanoliposomal formulation resulted in concentration-dependent changes in cell viability and cell death in both MCF-7 and HFF cells. The nanoliposomal formulation produced a greater cytotoxic effect against MCF-7 breast cancer cells than the free polyaniline&ndash;polyacrylic copolymer (<strong>P < 0.05</strong>). Flow cytometric analysis further demonstrated that both the free copolymer and the nanoliposomal formulation significantly promoted apoptotic cell death in MCF-7 cells, as evidenced by increases in the proportions of cells undergoing early and late apoptosis (<strong>P < 0.05</strong>). Moreover, treatment with the tested formulations resulted in increased expression of the caspase-3 and caspase-9 genes in MCF-7 cells, further supporting the involvement and activation of the intrinsic apoptotic pathway. Overall, these findings indicate that incorporation of the polyaniline&ndash;polyacrylic copolymer into nanoliposomes may enhance its anticancer activity and facilitate the induction of apoptosis in breast cancer cells.</span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="font-family:&quot;Times New Roman&quot;,serif"><strong>Conclusion:</strong> The findings of this study indicate that the polyaniline&ndash;polyacrylic copolymer possesses the potential to induce apoptotic cell death in MCF-7 breast cancer cells. Encapsulation of the copolymer within nanoliposomes appeared to enhance its cytotoxic and pro-apoptotic effects, suggesting that nanoliposomal delivery may represent a promising strategy for improving the anticancer potential of this polymeric compound. Further investigations are warranted to elucidate the underlying molecular mechanisms, optimize the nanoliposomal formulation, and evaluate its selectivity and therapeutic potential in additional in vitro and in vivo models.</span></span></span></span> نانولیپوزوم, کوپلیمر, خاصیت ضد سرطانی, خاصیت ضد میکروبی Polyaniline–polyacrylic copolymer, Nanoliposomes, MCF-7 breast cancer cells, Apoptosis, Caspase-3 0 0 http://aums.abzums.ac.ir/browse.php?a_code=A-10-1779-1&slc_lang=fa&sid=1 Ebrahim Balali ابراهیم بلالی ebrahimbalali2021@gmail.com 100319475328460015321 100319475328460015321 No Department of Organic Chemistry, TMS.C., Islamic Azad University, Tehran, Iran. 2 Medical Genomics Research Center, Tehran Medical Sciences Islamic Azad University, Tehran, Iran. گروه شیمی آلی، واحد علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Rezvan Fallahi ریژوان فلاحی rezhvan2000@gmail.com 100319475328460015322 100319475328460015322 No Department of Organic Chemistry, TMS.C., Islamic Azad University, Tehran, Iran. 2 Medical Genomics Research Center, Tehran Medical Sciences Islamic Azad University, Tehran, Iran. گروه شیمی آلی، واحد علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Seyyed Mohammad Esmail Mahboubi Rabbani سید محمد اسماعیل محبوبی ربانی mahbobi2021@gmail.com 100319475328460015323 100319475328460015323 No Department of Medicinal Chemistry, TMS.C., Islamic Azad University, Tehran, Iran. گروه شیمی دارویی، واحد علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Maryam Naser-ol-Eslami مریم ناصرالاسلامی naseresla2000@gmail.com 100319475328460015324 100319475328460015324 No Department of Molecular Cell Biology, TMS.C., Islamic Azad University, Tehran, Iran. گروه زیست شناسی سلولی مولکولی، واحد علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Ali Jebali علی جبالی alijebal2011@gmail.com 100319475328460015325 100319475328460015325 Yes Department of Nanotechnology, TMS.C., Islamic Azad University, Tehran, Iran گروه نانوتکنولوژی، واحد علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران