1 2322-3839 Alborz University of Medical sciences 797 Special Comparative Study of Biochemical and PCR Tests in the Detection of Mycoplasma Contaminations of Genital Secretions of Infertile Women and Men Hamedi Elnaz b Kheirkhah Babak c Amini Kumars d b Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran c Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran d Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran 1 8 2018 7 3 171 180 01 10 2018 01 10 2018 Introduction: Mycoplasma are from cell culture the main polluters. Mycoplasma hominis is one of the most important human mycoplasmas are associated with infections of the genitourinary system in women and men. The aim of this study compare methods of detection of mycoplasma contamination in the genital secretions of men and women referring to infertility center is Kerman. Materials and Methods: This descriptive cross-sectional study was performed on 100 women and 100 infertile men referred to Kerman Infertility Center during 6 months. Samples that were sent to the laboratory in the PPLO Broth filter 0.45 micrometers were passed and in accordance with the instructions, they were cultured in PPLO solid liquid and solid media and stored at 37 ° C,  Co2 incubator. After the extraction of DNA by using PCR method and especial primers, the species and genus of Mycoplasma hominis were determined and studied. Findings: Of the 200 samples examined by culture method, 17.5% of mycoplasma colonies were positive and using biochemical tests were mycoplasma hominis 6%. By PCR method, 44% of samples were infected with mycoplasma and 16.5% contaminated they were mycoplasma hominis. Discussion & Conclusion: The results of this study show that PCR is a sensitive and rapid method for detecting Mycoplasma huminis in infertile women and men.
798 Special A Review of Stem Cell Technology Mansouri Fatemeh e e Department of Genetics and Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran 1 8 2018 7 3 181 189 01 10 2018 01 10 2018 Stem cells as natural cells exist in embryonic and adult tissues. Recent studies have begun to prove the strong and powerful role of stem cells in the field of treatment. Stem cells have the potential for self-renewal and differentiation into specific types of somatic cells. Their ability to produce new cells in medicine, drug discovery, cell therapy and research is very important. In the past decades, many efforts have been made to find safe, low-cost methods and progress stem cell culture. The purpose of this review article is to: 1) explain the application of stem cells in medicine, drug discovery, modeling of disease and toxicology studies. 2) A summary of recent advances in stem cell technology, the advantages and disadvantages of the most commonly used culture methods. The result of this discussion shows that the use of new culture methods can be effective in the optimal use of stem cells. 799 Special Determining the Prevalence of qnrB Gene in Pseudomonas Aeruginosa Strains Isolated from Clinical Centers in Tehran in 1394 Salehi Mitra f Samani Sadaf g f Assistant Professor, Department of Microbiology, Azad University, North Branch, Tehran, Iran g MSc, Department of Microbiology, Azad University, North Branch, Tehran, Iran 1 8 2018 7 3 190 196 01 10 2018 01 10 2018 Background: Pseudomonas aeruginosa is one of the major causes of nosocomial infections. The rise in antibiotic resistance among Pseudomonas aeruginosa strains has led to many complications in treatment processes. Among commonly used drugs, resistance to Fluoroquinolones is especially of much importance. In this study the prevalence of qnrB gene was investigated in resistant Pseudomonas aeruginosa isolates. This gene is responsible for the development of resistance to Fluoroquinolones. Material and Methods: Pseudomonas aeruginosa isolates were collected from surfaces of clinical centers in Tehran and identified employing selective media and biochemical tests. Kirby Bauer Disk diffusion method was conducted for antibiotic susceptibility testing. The presence of qnrB gene was examined using polymerase chain reaction (PCR). Results: About %40 of the collected samples harbored Pseudomonas aeruginosa strains, %100 of which were resistant to nalidixic acid and %96 showed resistance to norfloxacin and ciprofloxacin. qnrB was present in %3.3 of the isolates. Conclusion: Analyzing the data from present study and previous research indicates that the rise in resistance rate is due to the extreme usage of Fluoroquinolones in clinical centers, apart from other prevailing reasons of this phenomenon. 800 Special Effect of Eight Weeks’ Resistance Training on Serum Levels of Irisin and Lipid Profile in Overweight Men’s with Nonalcoholic Fatty Liver Disease Nejadsalim Shahriyar h Gholami Mandana i Ghazaliyan Farshad j h Exercise physiology MSc From Islamic Azad University, Sciences and Research Branch, Tehran, Iran i Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran j Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran 1 8 2018 7 3 197 206 01 10 2018 01 10 2018 Background: Exercise training have a positive effect on nonalcoholic fatty liver disease (NAFLD). However, effectiveness pathways of exercise training on NAFLD is unknown. Therefore, the present study was conducted to aim of investigate the effect of eight weeks’ resistance training on serum levels of irisin and lipid profile in overweight men’s with nonalcoholic fatty liver disease. Methods: This study subjects consist of 30 overweight men’s afflicted by NAFLD with average age 40.2 ± 2.5 and weight 75.5 ± 3.19, that randomly assigned in control (n=15) and resistance training (n=15) groups. Resistance training program performed for eight weeks and three sessions in week. Training load from 40 percent of 1RM in first week increased to 70 percent of 1RM in last week. 48 hours after last exercise session, blood samples collected and given variables measured and data were analyzed by means of SPSS-24 software and analyze covariance (ANCOVA) test. Results: Results indicated significant increase of irisin and HDL (p=0.001) and significant decrease in LDL, TG and cholesterol (p=0.001) in resistance training group compared to control group. Conclusions: It seems that, some of positive effect of resistance training in patient with NAFLD can be attributed to increase irisin levels and resistance training can play important role in modulate lipid profile in this patient. 801 Special To Investigate the Abundance of Aluminum Phosphide Poisoning in Karaj-Iran from 2006 to 2011 Tavakoli-Far Bahareh k Mahdi Navesi Bahareh l Salehi Tannaz m Hosseini Asieh n Rahimzadeh Mitra o Dehghan-Tarzjani Mohammad Hossein p Abdollahi Mohammad Noorisepehr Mohammad k Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran l Food and Drug Administration, Alborz University of Medical Sciences, Karaj, Iran m Physiology Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran n Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran o Social Determinants of Health Research Center, Alborz University of Medical Sciences, Karaj, Iran p Department of Biochemistry Genetics Nutrition, Faculty of medicine, Alborz University of Medical Sciences, Karaj, Iran Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Department of Environmental Health Engineering, Faculty of Health, Alborz University of Medical Sciences, Karaj, Iran 1 8 2018 7 3 207 212 01 10 2018 01 10 2018 Background: Aluminium phosphide (ALP) is a highly effective insecticide and rodenticide used frequently to protect stored grain. Acute poisoning with this compound is common in some countries including India and Iran, and is a serious health problem. The objective of this study was to survey ALP poisoning and the outcome in hospitals in Karaj, Iran. Materials and Methods: The study was a retrospective study from April 2006 to April 2011. Records of all patients admitted and hospitalized to hospital during the five-year period were collected. Information including gender, age, amount of ALP consumed, route of exposure and outcome were extracted from the patients notes. Results: During the five-year period, 67 patients, 53.7% men and 46.3% women were admitted with ALP poisoning. Most poisoned people were under the age of 21 years.The most common signs and symptoms at admission were nausea (79.4%), vomiting (76.5%), abdominal pain (31.4%) and metabolic acidosis (41.1%). Suicidal intention was the most common cause of poisoning leading to 44.8% deaths and most cases were male (P<0/05). Conclusion: Due to high mortality, follow poisoning with ALP best treatment is prevention. It should be noted that although in previous years, hospitals did not record all cases of poisoning cases but data indicate a high consumption of this poisoning as a suicidal factor in Karaj city. Withdrawal of ALP tablet from the market and the monitoring of their performance can reduce the rate of poisoning in society. 802 Special The Effects of Taraxacum Officinale Alcoholic Extract on Serum Thyroid Hormone Levels in Adult Male Rat Dehghanian Razieh Hosseini Seyyed Ebrahim Sharifi Esfandiar Moghadamnia Davood M.Sc in Animal Physiology, Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Associated Professor in Animal Physiology, Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran Instructor, Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Ph.D in Animal Physiology, Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran 1 8 2018 7 3 213 219 01 10 2018 01 10 2018 Background: Taraxacum Officinale is a medical plant with antioxidant benefit effects. The aim of the present study is to evaluate the effect of Taraxacum Officinale alcoholic extract on serum thyroid hormone levels in adult male rat. Material and Methods: In this experimental study,45 adult male wistar rats were approximate average weight body of 200-250 gr were divided in to 5 groups of 9. Control ,sham ,and three experimental groups which received 50,100,150mg/kg of Taraxacum Officinale alcoholic extract by used gavage method. After this period (14 days) blood sample were collected and after measuring the amount of thyroid hormones of T3,T4,TSH hormones, data were analyzed by using SPSS software , ANOVA and t-test. Results: The results showed that body weight average and serum level of T4 hormone in receiving maximum dose of Taraxacum Officinale alcoholic extract showed significant decrease comparing to control group. T3 serum level in experimental groups receiving Taraxacum Officinale alcoholic extract didn't show any significant difference comparing to control group. TSH serum level in experimental groups receiving Taraxacum Officinale alcoholic extract showed significant decrease comparing to control group (p<0.05). Conclusion: The Taraxacum Officinale alcoholic extract reduced serum thyroid hormone levels in adult male rat. 803 Special Nano Drug Delivery Systems and Liposomes Goudarzvand Mahdi Ataie Zahra Department of Physiology and Pharmacology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran Department of Pharmaceutics, Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran 1 8 2018 7 3 220 232 01 10 2018 01 10 2018 Progressive development of nanotechnology during the last decades and its increasing involvement in new fields represents its great importance in science and technology. One of its major parts is nanomedicine that is focused in this review. First general principles of medicinal nanotechnology, its history, application and classification are explained. Then Abraxane (a protein conjugated nano drug) design will be addressed. Liposomes as one of the best nano drug delivery systems with a platform for passive targeting are discussed next. Two examples of approved liposomal nanomedicines are described from the state-of-the-art design emphasizing physiologic barriers and formulation strategies to overcome these imposed limitations. Passive targeting to tumors is provided by "Enhanced Permeation and Retention" effect (EPR). Liposomes can be removed from circulation by the mononuclear phagocyte system (MPS) clearance in no time. This effect is also used to passively target liposomes in MPS to treat localized infections for example. Pegylation of liposomes which means attachment of polyethylene glycol chains to the surface of liposomes is one of the methods used to enhance liposome residence time in blood circulation in order to give the vesicles more chance to accumulate at the target site by EPR effect. Liposomal medicines have shown significant decrease in toxicity in comparison with free drugs. Their accumulation in target tissue by passive targeting is the main reason for reduction in drug toxicity.   Liposomes are being used for treating different diseases such as infection, hepatitis, cardiovascular diseases and immune system disorders. However, the vast majority of investigational liposomes are anticancer drugs. 804 Special Effects of Olibanum Extract on Differentiation of Bone Marrow Stem Cells to Neurons Kermanian Fatemeh Mansouri Mohammad Reza Zamani Shokofeh Pahlavan Shahrzad Shamosi Atefeh Department of Anatomy, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Students Research Committee, Alborz University of Medical Sciences, Karaj, Iran Department of Internal Disease, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Pathology Lab, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Department of Anatomy, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran 1 8 2018 7 3 233 242 01 10 2018 01 10 2018 Introduction: Central nervous system has the low potential for regeneration of damaged neurons. The lack of suitable treatments for neurodegenerative diseases has placed the huge financial burden on the community. Stem cells have self-renewing capabilities and can differentiate into adult cell types under the appropriate conditions. The chemical properties of some plants affect the growth rate and cell proliferation. Previous studies have shown that Olibanum plant plays a role in the formation of dendritic spines and neuronal regeneration. In the present study, the effect of olibanum on the differentiation of bone marrow-derived stem cells (BMSCs) to neuron was investigated. Material and Methods: Bone marrow stem cells were cultured in a Dulbecco's Modified Eagle's Medium, 10% fetal bovine serum, Fibroblast growth factor 2, and Epidermal growth factor (DMEM, FBS, FGF2, and EGF). Olibanum extract 5%, 10%, and 20% were added to culture medium. Then microscopic observations, MTT assay, and immunocytochemistry examination were used to assess morphological, proliferation and differentiation factors. Results: Microscopic observations and viability cell test showed that the 5% Olibanum extract was non-toxic to BMSCs. Immunocytochemistry result denoted that 5% Olibanum extract can increase the differentiation of BMSCs into neuron-like cells. However, when Olibanum extract 10% and 20% were added to the culture medium, such an increase was not seen in treated groups. Conclusion: These findings suggest that the application of 5% Olibanum extract in cell culture medium was shown to have a suitable effect on BMSCs differentiation into the neuron. 805 Special Salt Powder in Management of Umbilical Granuloma: A Randomized Trial Study Golshan Mohammad Darijani Azam Assistant Professor, Pediatric Department of Azad University of Medical Sciences and Health Services, Tehran, Iran General Physician, Pediatric Department of Azad University of Medical Sciences and Health Services, Tehran, Iran 1 8 2018 7 3 243 247 01 10 2018 01 10 2018 Background: Alcohol, antibiotic ointments, silver nitrate and ligation had been presented for treatment of umbilical granuloma. Salt powder due to its hyper osmolarity effects can induce drying and necrosis of granuloma tissue. Present parallel single blind randomized clinical trial was performed in neonates of shahvali hospital for assessment of salt powder in management of umbilical granuloma. Patients and Methods: Present parallel single blinded randomized clinical trial was performed on 60 neonates with umbilical granuloma between January and October 2015. Our samples were selected randomly from neonates with healthy umbilical granuloma. Neonates in case group, salt powder was prescribed in five days (30 minutes two times per day) and located on the umbilical granuloma. During these five days neonates in control group were only clinically examined. Complete removal of umbilical granuloma was considered as study outcome. Results: Umbilical granuloma removed in all neonates of case group who were treated with salt powder but in control group, only four neonates had complete granuloma removing. Recovery rate had significant difference between two trial groups (p=0.00). Conclusion: Management of umbilical granuloma with salt powder in neonates had suitable therapeutic effects in comparison with other methods without any complications. 806 Special The Effectiveness of Schema Therapy Based on Acceptance and Mindfulness in Intimacy, Adaptation and Resiliency in Persons with Marital Conflicts in Couples of City Karaj Alizadeh Asli Afsaneh Jafar Nezhad Langroudi Zeinab Department of Psychology, Buinzahra Branch, Islamic Azad University, Buinzahra, Iran Department of Psychology, Buinzahra Branch, Islamic Azad University, Buinzahra, Iran 1 8 2018 7 3 248 256 01 10 2018 01 10 2018 Introduction: The aim of this study was to determine the efficacy of schema therapy based on admission enrichment program and mindfulness in intimacy, adaptability and resilience in women with marital conflicts in Karaj. Materials and Methods: The research method was quasi-experimental. The statistical population of the study was all couples with marital conflicts referring to Karaj health centers. The sampling method was available in this study. In this method, 30 women with marital conflicts were selected and randomly assigned into two experimental and control groups (each group was 15). Then, the experimental group participated in 12 sessions of 90 minutes of schema therapy based on the admission and mindfulness communication enrichment program in a group manner, and the control group spent its daily routine. Data were collected using marital adjustment, resilience, marital conflicts and marital intimacy. Data were analyzed using covariance analysis and SPSS software. Results: The results showed that the acceptance schema therapy based on the acceptance and mindfulness enrichment program significantly increased intimacy, compatibility and resilience and reduced marital conflicts in the experimental group (p<0.50). Conclusion: Based on the results, we can conclude that the schema therapy based on the program of communication enrichment acceptance and mind-awareness in order to increase intimacy, compatibility and resilience, as well as reduction of marital conflicts.