per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
257
265
article
Characterization of Phototherapy at Home in Neonatal with Jaundice
Mohammad Golshan Tafti
mgolshan035@gmail.com
1
Sara Golzari
2
Assistant Professor of Islamic Azad University Branch, Yazd Medical School of Ali ebn abi taleb, Yazd, Iran
Medical Student of Islamic Azad University, Branch Yazd Medical School of Ali ebn abi taleb, Yazd, Iran
Objective: Neonatal jaundice is one of the most common findings in examination of neonates. Phototherapy is a health and safe procedure as well as one of the most common methods for treating of indirect hyperbilirubinemia. The aim of this study was to compare characteristics of home phototherapy with hospital phototherapy in jaundiced neonates.
Material and Methods: This research was a cohort study. This study was carried out on 120 term(and older than 24 hours) neonates who was admitted to the pediatric ward of Shohadaye Kargar Hospital, Yazd, Iran and also the refereed neonates to Yazd Isatis Emdad for phototherapy. The neonates was divided into 2 groups (home phototherapy and hospital phototherapy), randomly. Phototherapy was performed in both groups. Serum bilirubin was measured and recorded in neonates in the beginning of phototherapy and after 24 hour. Data was collected and analyzed by appropriate statistical tests using SPSS software version 17.
Results: According to the present study, the average serum bilirubin level in home phototherapy and hospital phototherapy was 10.52 and 10.97 respectively. (SD 2.09 vs. 1.72). Bilirubin decreased 3.84 mg/dl in home phototherapy and 3.73 mg/dl in hospital phototherapy. The average duration of phototherapy at home was lower than hospital phototherapy. In addition, average cost of phototherapy at home was lower than hospital phototherapy. Dehydration was not obvious in the study duration. Diarrhea was found in 2 neonates at home and 6 neonates in hospital during phototherapy. During phototherapy, 31 neonates at home and 38 neonates in hospital suffered skin rash. Satisfaction rate was 92.3% in hope phototherapy and 70% in hospital phototherapy.
Conclusion: According to this study, age, gender, weight at birth and phototherapy complications were equal in home phototherapy and hospital phototherapy. Decreased level of bilirubin in home phototherapy was same to hospital phototherapy. Moreover, Duration of phototherapy and costs in home group were lower than hospital group, significantly.
http://aums.abzums.ac.ir/article-1-848-en.pdf
Neonatal jaundice
Home phototherapy
Hyperbilirubinemia
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
266
280
article
Effect of Propranolol in Human Body Using Gold Nanoparticles by Spectrofluorometric Method
Ali Akbar Shadbahr
1
Farzaneh Marahel
farzanehmarahel@yahoo.com
2
Chemistry Department, Faculty of Sciences, Islamic azad University, Omidiyeh Branch, Omidiyeh, Iran
Chemistry Department, Faculty of Sciences, Islamic azad University, Omidiyeh Branch, Omidiyeh, Iran
In this method, gold nanoparticles have been synthesized by reducing the gold salt and the Spectrofluorometric spectrum is taken from it. Determination of propranolol is widely used in the treatment of blood pressure and irregular heart beat .The effects of different parameters such as pH; volume of buffer; volume of gold nanoparticles and remaining time are investigated. The results showed that the optimal pH value of 6, 2.5 milliliters as the optimum buffer volume, the optimal volume of gold nanoparticles was 3 milliliters and 6 minutes as the optimum remaining time was determined for specimens and following it the response of the nanoparticles to the drug has been plotted. After determining the calibration curve, it was determined that a very wide linear range of drug can be investigated using this method. The method has been found to have appropriate figure merits. At the end of the study, golden Nano sensor was used in real samples such as blood and urine to measure propranolol and obtained satisfactory results.
http://aums.abzums.ac.ir/article-1-849-en.pdf
Gold nanoparticles
Propranolol drug
Spectrofluorometric device
Gold salt
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
281
292
article
The Effects of Seaweed Gracilaria arcuata Extract on the Stimulation of Apoptosis in Colorectal Cancer Cell Lines
Ali Taheri
taherienator@gmail.com
1
Mostafa Ghaffari
2
Shadab
Houshmandi
3
Mehdi Namavari
4
Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
Razi Vaccine and Serum Research Institute, Education and Extension Organization (AREEO), Shiraz, Iran
Introduction: The most common problems in the medical sciences is resistance of cancer cells to anticancer drugs. Thus, finding new anti-cancer agents with minimal side effects seem necessary. In this regard, different studies on different marine algae are reported. This study aimed to investigate the effects of the algae Gracilaria arcuata extract on the stimulation of apoptosis in colorectal cancer cell lines and the effects on cell DNA fragmentation. This study was conducted as an In vitro study.
Materials and Methods: The anticancer effects of aqueous and organic extracts of algae were tested by the MTT Assay, Trypan blue staining, apoptosis by flow cytometry and DNA fragmentation assay methods.
Results: The results of this study imply that the anti-cancer properties tested by MTT and Trypan blue assay, showed that methanol extract of Gracilaria arcuata with 44.45 ± 0.91 µg/mL concentration had the maximum effect on cancer cell death and the minimum effect was seen in the hexane (70.22 ± 1.22 µg/mL) and ethyl acetate extracts (70.55 ± 2.67 µg/mL), respectively (p <0.05). Results of trypan blue test confirmed the results of the MTT test, but the survival rate were different. To determine the effect of the algae methanol extract, cell apoptosis was assayed by two methods of flow cytometry and DNA fragmentation. Algae extracts of Gracilaria arcuata have LC50 in the concentration of 1010.16 µg/mL and apoptosis of 7.5 percent, which showed little apoptotic effect.
Conclusion: The results show that the algae Gracilaria arcuata extract had anticancer effects against colorectal cancer cell lines, but additional studies of the chemical structure of bioactive compounds are needed.
http://aums.abzums.ac.ir/article-1-850-en.pdf
Anti-cancer
Gracilaria arcuata
Colorectal cancer
Apoptosis
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
293
298
article
Clinical and Epidemiologic Findings of Pertussis Suspected Patients Referred to Zanjan Health Centers in 2011-2012
Mojtaba Kamali Aghdam
1
Alinaghi Kazemi
2
Farmehr Elmi
3
Mansour Sadeghzadeh
sadeghzadeh@zums.ac.ir
4
Department of Pediatrics, Zanjan University of Medical Sciences, Zanjan, Iran
Department of Pediatrics, Zanjan Metabolic Disease Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
Zanjan University of Medical Sciences, Zanjan, Iran
Department of Pediatrics, Zanjan Metabolic Disease Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
Introduction: Despite frequent vaccination, Pertussis is still relatively common in the world. The prevalence of this disease has been reported differently in different parts of the world. The aim of this study was to investigate clinical and pediatric findings and compare them with the results of nasopharyngeal culture in suspected Pertussis patients in Zanjan province.
Materials and Methods: In this cross-sectional study, records of all patients referred to Zanjan health center during 2011-2015 with suspected Pertussis were evaluated. Data were collected from questionnaires and analyzed by SPSS v16 software using frequency distribution, chi-square and chi-square test.
Findings: Of the 243 patients examined, 17 (7%) patients were positive for nasopharyngeal culture. Eight of them were male and 9 were females. The highest number of cases was in winter (9 cases). We found cough in all cases and post coughing vomiting in 15 cases . Of the 17 positive cultured patients, 11 patients were below 6 months (before completing three doses of vaccine).
Discussion & Conclusion: In this study, the positive nasopharyngeal culture was similar to most of the national’s studies but was less than that of other countries.
http://aums.abzums.ac.ir/article-1-851-en.pdf
Pertussis
positive culture of nasopharynx
Zanjan
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
299
308
article
Identification and Comparison of Methicillin Resistance mecA Gene in Staphylococcus Aureus Isolated from Clinical Specimens Before and After Open Heart Surgery in Shariati Hospital in Tehran and in Dey Private Hospital
Marziyeh Sadat Amini
amini90_13@yahoo.com
1
Faramarz Soleymanloo
2
Sajad Alizadeh
3
Hadi Feizi
4
Yashar Bagherizadeh
5
Mohammad Movagharnezhad
6
Mehdi Jahangiri-Hoseinabadi
7
Department of Microbiology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Head of Laboratory Dey Hospital, Tehran, Iran
Department of Microbiology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Department of Microbiology, zanjan Branch, Islamic Azad University, zanjan, Iran
Department of Microbiology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Department of Microbiology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Department of Microbiology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Background and Aim: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of hospital infections. MRSA is resistant to all beta-lactams due to the presence of the mecA gene. The aim of this study was to isolate and identify the methicillin resistance gene of Staphylococcus aureus by PCR technique, before and after open heart surgery in Day and Shariati hospital.
Materials and Methods: After collecting samples, various biochemical and microbiological tests were performed and then antibiotic susceptibility test using disc diffusion method based on CLSI instructions with antibiotics from different groups. It were used turned out to determine the resistance gene, PCR and Qpcr.
Results: The results showed that the prevalence of mecA gene in clinical samples was 30% and there was a significant difference in the frequency of isolates of Staphylococcus aureus and MRSA in two hospitals of Day and Shariati. Among the antibiotics, the resistance before the operation of the open heart surgery was 95% for the oxacillin antibiotic and the antibiotic resistance after the surgery was higher than that for oxacillin 100%, erythromycin and, Ceftazidime 97%, which was tested more than the other antibiotics.
Conclusion: Because of the importance of Staphylococcus aureus as the most important pathogenic pathogen and considering the significant reduction of MRSA isolation after open heart surgery in the private hospital of Day in comparison with Shariati Hospital, It can be concluded that it is likely that the environmental conditions and the principles of sterilization in private hospitals are much better than government hospitals.
http://aums.abzums.ac.ir/article-1-852-en.pdf
Staphylococcus aureus
Antibiogram
mecA
PCR
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
309
314
article
Investigating the Ability of Producing Biofilm by Isolated Salmonella from Food
Abolfazl Sirdani
1
Mohammad Mehdi Soltan Dallal
msoltandallal@gmail.com
2
Department of Microbiology, Faculty of Life Sciences, Islamic Azad University of Damghan, Damghan, Iran
Food Microbiology Research Center / Division of Food Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Introduction: Food-borne disease has always been a threat to human health and is considered as a matter of urgency worldwide. Many outbreaks have been associated with biofilms. In addition, Biofilm has been considered as a problem in the food industry because of quick resistance to anti-microbial and cleansing agents. Biofilm formation cause pathogenic effect in Salmonella, especially in the food industry, which allows bacteria to bind to different surface. The aim of this study was to investigate the ability of producing biofilm by isolated Salmonella from food
Materials and Methods: Eight Salmonella isolated from 400 samples of red meat, chicken, egg and vegetable were identified by conventional biochemical and serotype tests. Their ability to produce biofilm was measured using titration method in microplate crystal violet.
Results: In the phenotypic study of Salmonella isolates with 550 nm doping control method and without acetic acid, only 2 isolates (25%) had the ability to produce biofilm, both of which belong to the group D of Salmonella.
Conclusion: Considering the ability to create biofilms of Salmonella isolated from food and increasing their antibiotic resistance, and increasing gastroenteritis caused by Salmonella, especially serogroup D, more care and higher levels of health in the provision, production, food packaging and delivery are required.
http://aums.abzums.ac.ir/article-1-856-en.pdf
Salmonella
Biofilm
Food-borne diseases
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
315
322
article
The Relationship Between General Health and Marital Satisfaction in Married Women Referred to Health Centers in Karaj
Mansooreh Tajvidi
mansooreh_tajvidi@yahoo.com
1
Tooran Bahrami
2
Department of Nursing, College of Nursing and Midwifery, Karaj Branch, Islamic Azad University, Alborz, Iran
Faculty Member of Social Determinants of Health Research Center, Alborz University of Medical Sciences, Karaj, Iran
Background and Aims: According to the importance of marital satisfaction in family functioning as a system that is directly reflected in the functioning of family members, it is important that we understand the factors in predicting and enhancing sexual satisfaction. This study aimed to investigate the relationship between general health and marital satisfaction.
Method: The research was cross-sectional and correlational study, 122 married women referred to health centers in Karaj using a convenience sampling participated in the study. Enrich marital satisfaction questionnaire and general health questionnaire were used. To analyze the data was used SPSS version 16.
Results: The results showed there is a significant correlation between marital satisfaction and general health in married women (p<0.001).
There is a significant relationship between the mean score of marital satisfaction and age, age of husband, husband's and wife's education, (p <0.05).
Conclusion: According to the important role of marital satisfaction on the strong foundation of the family and its relationship to public health, Health professionals should apply solutions to enhance the general health of married women.
http://aums.abzums.ac.ir/article-1-857-en.pdf
Health
Marital status
women
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
323
332
article
Survey of Integron Types and Carbapenem Resistance Encoding Genes in Acinetobacter Baumannii Isolated from Burn Wound Samples
Samaneh Saeedi
1
Mohammad Reza Abdolsalehi
2
Mahmoud Khodabandeh
3
Azadeh Alvandimanesh
4
Abazar Pournajaf
5
Ramazan Rajabnia
ramazan69@yahoo.com
6
Department of Microbiology, School of Basic Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
Department of Pathology, Shafa Hospital, Qhazvin University of Medical Sciences, Qhazvin, Iran
Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
Introduction: Acinetobacter baumannii is one of the most important pathogenic bacteria that causes nosocomial infections, especially in burned patients. In recent years, Carbapenems have been considered as a selective antibiotic in the treatment of multi-drug resistant A. baumannii, but the rapid appearance of carbapenem-resistant A. baumannii has been reported worldwide. The purpose of this study was to investigate different classes of integron and Carbapenem-resistance encoding genes in A. baumannii isolated from burn wound samples in Shahid Motahari Hospital in Tehran.
Material and Methods: In this descriptive cross-sectional study, 70 samples of A. baumannii were collected from burn injured patients referred to the Motahari Hospital. After identification and confirmation of strains, an antibiotic susceptibility test was performed using disk diffusion agar test. Also, metallo-β-lactamases producing isolates were identified using combined disk and E-test. BlaKPC producing strains were also identified using modified Hodge test. Finally, in order to evaluate genomic strains, after DNA extraction using boiling method, the PCR reaction for intI, intII, intIII, IMP, VIM, NDM, OXA-23-like, OXA-24-like, OXA genes -85-like, OXA-51-like and KPC.
Results: In this study, all A. baumannii isolates were resistant to ciprofloxacin, cefotaxime, piperacillin and Trimethoprim/sulfamethoxazole. Out of 67 imipenem-resistant isolates, 57 (85.1%) and 61 (91%) isolates were considered as MBL producing in combination phenotypic tests and E-test. 44 (65.6%), 21 (31.3%) and 1 (1.5%) isolates were positive for blaVIM, blaIMP and blaNDM genes, respectively. So, 52 (77.6%) and 27 (40.3%) had OXA-23 and OXA-24 genes. 11 (15.7%) isolates were positive for KPC production in the Modified Hodge Test, but only 27.3% (3 strains out of 11 isolates) were carrying blaKPC gene. Also, 12 (17.1%), 54 (77.1) and 3 (4.3%) isolates, were carrying intI, intII and intIII genes, respectively.
Conclusion: The increased frequency of carbapenem-resistant A. baumannii in burn patients suggests choosing an appropriate antibiotic regimen based on the antibiotic susceptibility pattern of the isolates. The rapid identification of carbapenemase-producing strains is helpful to select suitable options for antimicrobial therapy and prevent the further spread of their encoding genes.
http://aums.abzums.ac.ir/article-1-853-en.pdf
Integrone
carbapenemase genes
A. baumannii
Modified Hodge Test
burn
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
333
344
article
Design of the Pattern of Effective Environmental Factors For Outsourcing the Services in Public Hospitals
Morteza Nazari
1
Leila Riahi
l.riahi@srbiau.ac.ir
2
Seied Jamaleddin Tabibi
3
Mahmoud Mahmoudi Majdabadi Farahani
4
PhD Student of Health Services Management, Science and Research Branch, Islamic Azad University, Tehran, Iran
Assistant Professor, Department of Health Services Management, Science and Research branch, Islamic Azad University, Tehran, Iran
Professor, Department of Health Services Management, Science and Research Branch, Islamic Azad University, Tehran, Iran
Professor, Department of Health Services Management, Science and Research Branch, Islamic Azad University, Tehran, Iran
Background: Hospitals are considered as the most important consumer units in healthcare and one of the main organizations of health services. Therefore, paying attention to their performance and effectiveness is of special importance. The aim of this study is to design an effective and efficient pattern for determination of effective environmental factors to outsource the services in public hospitals of country to increase the success rate in delivering the health services and improve the performance level and increase productivity by using them.
Materials and Methods: Considering the results, this is an applied study and has an exploratory purpose. A questionnaire with 20 questions has been used for data collection, the variables of which are derived from comparative studies, its validity has been evaluated by the experts and its Cronbach's alpha coefficient was %86 in final measurement. The research community of the study was science committee of the universities, health system policy makers, experts in outsourcing and public hospital managers. Stratified sampling method was used for sampling. Data was analyzed by IBM SPSS statics 23 and LISREL 8.8.
Findings: According to collected information, Important environmental factors on outsourcing were policy factor with the average of 3.67%, risk assessment 3.38, background 3.25 and development 2.83, in order of importance. The average of total score of environmental factor effect was 3.21 and was meaningfully more than the average amount of 3 (p<0.001). It shows that environmental factors are of important factors in outsourcing the hospital services.
Conclusion: For achieving the desirable goals in outsourcing the services and avoiding the assignment problems, it is important to evaluate the external environment of hospital carefully based on the extracted components from this study and using an appropriate pattern for decision making in outsourcing the services.
http://aums.abzums.ac.ir/article-1-854-en.pdf
Hospitals
Outsourcing
Risk assessment
per
Alborz University of Medical sciences
Alborz University Medical Journal
2322-3839
2588-3046
2018-12
7
4
345
351
article
Prevalence of Gram-Negative Bacteria Among Patients Admitted to Shahid Rajaee Hospital of Karaj in 1396
Sara Ghozati
1
Fatemeh Noorbakhsh
niloofar_noorbakhsh@yahoo.com
2
Ali Ehsan Heidary
3
Department of Microbiology, Biological Science College, Varamin-Pishva branch, Islamic Azad University, Varamin-Pishva, Iran
Department of Microbiology, Biological Science College, Varamin-Pishva branch, Islamic Azad University, Varamin-Pishva, Iran
Department of Pathobiology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran
Introduction: Septicemia or blood infection is one of the most important causes of mortality in patients hospitalized in different parts of hospitals, which is considered as an emergency medical emergency. This study was conducted to investigate the role of Gram-negative bacteria in the development of blood infection in Rokhayee hospital in Karaj.
Materials and Methods: This study was performed on 3500 patients with septicemia suspicious symptoms admitted to Shahid Rajaee Hospital. Samples were taken from patients for blood culture. The bacteria developed in blood cultures were isolated and identified using conventional bacteriological methods.
Results: Out of 3500 patients under study during one year, 70 cases of Gram-negative bacteria were isolated in blood cultures, 13 of which were in the first six months of the year and 57 of them were in the second six months. In total, six types of Gram-negative bacteria were isolated from patients (Escherichia coli, Enterobacter, Pseudomonas, Acinetobacter, Yersinia, and Klebsiella). Escherichia coli was the most common bacteria responsible for the infection of the blood.
Conclusion: The results of this study showed that Gram-negative bacteria were the main causes of blood infection and mortality and the prevalence of blood infections in the second month was higher.
http://aums.abzums.ac.ir/article-1-855-en.pdf
Septicemia
Bacteremia
Hospital infection
Hospitalized patients