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:: Volume 5, Issue 2 (5-2016) ::
aumj 2016, 5(2): 123-128 Back to browse issues page
Effect of Amniotic Membrane Homogenate on Ischemic Skin Flap Survival in Rat Model
Gh. Khorasani , D. Nazemisalman , A. Atri , K. Ghanbarzadeh , N. Rahmanpanah
Department of Plastic Surgery,Imam Khomeini Hospital,Tehran University of Medical Sciences,Iran , rezadns@yahoo.com
Abstract:   (950 Views)

Bachground: Surgical skin flaps are frequently used in plastic and reconstructive surgery to repair acquired or congenital defects. Necrosis is a common complication associated with these flaps postoperatively as a result of inadequate blood supply. This study was designed to evaluate the effectiveness of amniotic membrane homogenate in healing the dorsal skin of the rat as an ischemic flap model.

Materials and Methods: In this experimental study, twenty male Wistar rats weighing between 350 and 400 g were used in the study. For all rats 3*10 cm, full-thickness, caudally based pattern dorsal skin flaps were elevated. The rats were randomized into 2 groups: control (I) and experimental (II). Then in 10 point,0.1 mL amniotic membrane homogenate was injected with with equal interval in experimental group and normal salin administered in control group. Seven days later all skin flaps were examined mechanically (necrosis) and histologically.

Results: Tissue flap necrosis showed significantly reduced in amniotic membrane homogenate group (p=0.032), and tissue flap survival showed significantly increased in amniotic membrane homogenate group (p=0.036). There were no significant differences in the histology analysis heling proccess between two groups (p>0.05).

Conclusion: It seems that, the administration of amniotic membrane homogenate is an effective and safe method that improves the survival of ischemic skin flaps in a rat model.

Keywords: Ischemic skin flaps, Amniotic membrane homogenate, Survival, Necrosis
Full-Text [PDF 187 kb]   (520 Downloads)    
Type of Study: Research | Subject: Special
Received: 2016/06/11 | Accepted: 2016/06/11 | Published: 2016/06/11
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khorasani G, nazemisalman D, atri A, ghanbarzadeh K, rahmanpanah N. Effect of Amniotic Membrane Homogenate on Ischemic Skin Flap Survival in Rat Model. aumj. 2016; 5 (2) :123-128
URL: http://aums.abzums.ac.ir/article-1-423-en.html

Volume 5, Issue 2 (5-2016) Back to browse issues page
نشریه دانشگاه علوم پزشکی البرز Alborz University Medical Journal
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