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Determining the Prevalence of qnrB Gene in Pseudomonas Aeruginosa Strains Isolated from Clinical Centers in Tehran in 1394
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Mitra Salehi1   , Sadaf Samani *  2 |
1- Assistant Professor, Department of Microbiology, Azad University, North Branch, Tehran, Iran
2- MSc, Department of Microbiology, Azad University, North Branch, Tehran, Iran , sadafsamani68@gmail.com |
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Abstract: (2992 Views) |
Background: Pseudomonas aeruginosa is one of the major causes of nosocomial infections. The rise in antibiotic resistance among Pseudomonas aeruginosa strains has led to many complications in treatment processes. Among commonly used drugs, resistance to Fluoroquinolones is especially of much importance. In this study the prevalence of qnrB gene was investigated in resistant Pseudomonas aeruginosa isolates. This gene is responsible for the development of resistance to Fluoroquinolones.
Material and Methods: Pseudomonas aeruginosa isolates were collected from surfaces of clinical centers in Tehran and identified employing selective media and biochemical tests. Kirby Bauer Disk diffusion method was conducted for antibiotic susceptibility testing. The presence of qnrB gene was examined using polymerase chain reaction (PCR).
Results: About %40 of the collected samples harbored Pseudomonas aeruginosa strains, %100 of which were resistant to nalidixic acid and %96 showed resistance to norfloxacin and ciprofloxacin. qnrB was present in %3.3 of the isolates.
Conclusion: Analyzing the data from present study and previous research indicates that the rise in resistance rate is due to the extreme usage of Fluoroquinolones in clinical centers, apart from other prevailing reasons of this phenomenon. |
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| Keywords: Pseudomonas aeruginosa, qnrB gene, Fluoroquinolones |
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Full-Text [PDF 215 kb]
(1577 Downloads)
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Type of Study: case report |
Subject:
Special
Received: 2018/10/01 | Accepted: 2018/10/01 | Published: 2018/10/01
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