Background: Treatment of hydatidosis in humans, mainly surgery with the use of drugs is combined. During surgery, the risk of fluid leakage hydatid cyst that was content to adjacent tissues, resulting in recurrence of the disease. Ephedra is a plant that grows in various regions of Iran and used to treat as antibacterial and antifungal in certain diseases. The aim of this study was to in vitro evaluate the effects of different concentrations of aqueous extract of Ephedra major at different times.

Materials and Methods: Infected liver to hydatid cysts were collected from slaughterhouses. Protoscoleces viability was assessed by staining Eosin 1.0 percent. Protoscoleces activities of aqueous extract 1/0, 01/0, 001/0 mg/ml at 10, 20, 30 and 60 minutes were analyzed. Saline and saturated brine were used as positive and negative controls.

Results: The highest percentage of household protoscoleces dilution of 0.1 (13.58%) was observed (P= 0.001). Protoscoleces lowest scolicidal effect rate was found in the dilution 0.001 (2.47%)

Conclusions: The effect of aqueous extract of Ephedra major plumbing protoscoleces not significant. Therefore, it cannot be used as a cache protoscoleces strong and appropriate.